大豆根边缘细胞对土荆芥组培根分泌物的响应  

Response of root border cells of soybean to cultured Chenopodium ambrosioides root exudates

作　　者：李安奇[1] 王亚男[1] 张红[1] 汪利沙[1] 马丹炜[1] 

机构地区：[1]四川师范大学生命科学学院,四川成都610101

出　　处：《生态环境学报》2012年第1期84-87,共4页Ecology and Environmental Sciences

基　　金：四川省应用基础项目(2009JY0065);四川师范大学校级项目(08KYL05;10MSL01);四川师范大学研究生科研创新基金项目(校研字[2011]3号)

摘　　要：运用组织培养技术和悬空气培养法,研究了大豆根边缘细胞对土荆芥组培根分泌物的响应。结果表明:在土荆芥组培根分泌物作用下,随处理浓度的升高和处理时间的延长,边缘细胞数量和存活率持续下降。游离的根边缘细胞周围聚集了一些颗粒状物质,根边缘细胞可能螯合土荆芥根系分泌物。土荆芥根系分泌物能促进根冠果胶甲基酯酶活性上调,但是随着处理浓度增大和处理时间延长,这种促进效应降低。这些结果表明土荆芥组培根分泌物对大豆根边缘细胞具有一定的胁迫效应,根边缘细胞通过加速死亡并分泌一些活性物质螯合土荆芥根系分泌物,可在一定范围内缓解根系分泌物的毒害作用。By using the techniques of tissue culture and aeroponic culture, response of root border cells of soybean （ Giycine max L. ） to cultured Chenopodium ambrosioides root exudates was studied. The results showed the number and activity of root border cells decreased consecutively when treated with cultured C. ambrosioides root exudates. Some granules gathered around free root border cells would result from chelation between root border cells exudates with that of the cultured root. The cultured root exudates could stimulate the activity of pectin methylesterase of the root cap of soybean, but the effect gradually lowered down along with the increasing of treatment concentration and treatment time. The results suggested that the cultured root exudates had stress on root border cells of soybean and root border cells of soybean alleviated the allelopathic effects of the cultured root exudates on root tips though programmed cell death and chelating.

关 键 词：土荆芥 根分泌物 边缘细胞 果胶甲基酯酶 

分 类 号：Q948[生物学—植物学]