MicroRNA-125b Induces Cancer Cell Apoptosis Through Suppression of Bcl-2 Expression  

作　　者：Aihua Zhao Quan Zeng Xiaoyan Xie Junnian Zhou Wen Yue Yali Li Xuetao Pei 

机构地区：[1]Department of Obstetrics and Gynecology,General Hospital of PLA,Beijing 100853,China [2]Stem Cell and Regenerative Medicine Lab,Beijing Institute of Transfusion Medicine,Beijing 100850,China [3]Department of Obstetrics and Gynecology,The 309th Hospital of PLA,Beijing 100091,China

出　　处：《Journal of Genetics and Genomics》2012年第1期29-35,共7页遗传学报（英文版）

基　　金：supported by the National High Technology Research and Development Program of China(No. 2006AA02A107);the Major State Basic Research Program of China(Nos.2009CB521704 and 2010CB945504);the National Nature Science Foundation of China(No.30873031)

摘　　要：MicroRNAs （miRNAs） are small, noncoding RNAs which can often act as an oncogene or a tumor suppressor. Several miRNAs are associated with the development of hepatoceUular carcinoma （HCC）. We demonstrated that miR-125b significantly suppresses HCC cell proliferation and promotes apoptosis by inhibiting the gene expression of the anti-apoptotic protein, Bcl-2. Bioinformatic analysis indicated that the 3tUTR of Bcl-2 has binding sites for miR-I25b, Luciferase reporter assay confirmed the ability of miR-125b to dramatically suppress Bcl-2 transcription, suggesting that Bcl-2 is a target gene for miR-125b. We concluded that miR-125b acts as a tumor suppressor in hepatic tumor development by targeting Bcl-2 and inducing cancer celt apoptosis.MicroRNAs （miRNAs） are small, noncoding RNAs which can often act as an oncogene or a tumor suppressor. Several miRNAs are associated with the development of hepatoceUular carcinoma （HCC）. We demonstrated that miR-125b significantly suppresses HCC cell proliferation and promotes apoptosis by inhibiting the gene expression of the anti-apoptotic protein, Bcl-2. Bioinformatic analysis indicated that the 3tUTR of Bcl-2 has binding sites for miR-I25b, Luciferase reporter assay confirmed the ability of miR-125b to dramatically suppress Bcl-2 transcription, suggesting that Bcl-2 is a target gene for miR-125b. We concluded that miR-125b acts as a tumor suppressor in hepatic tumor development by targeting Bcl-2 and inducing cancer celt apoptosis.

关 键 词：miR-125b BCL-2 APOPTOSIS PROLIFERATION 

分 类 号：Q255[生物学—细胞生物学] X503.1[环境科学与工程—环境工程]