亚砷酸联合硼替佐米对肝癌细胞系BEL-7402的作用  

Bortezomib enhances arsenous acid-induced growth inhibition and apoptosis of BEL-7402 cells

作　　者：刘婷[1] 刘静[1] 褚艳杰[1] 胡丽红[1] 刘冰熔[1] 

机构地区：[1]哈尔滨医科大学附属第二医院消化内科,黑龙江省哈尔滨市150086

出　　处：《世界华人消化杂志》2012年第1期9-14,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30871152~~

摘　　要：目的:探索亚砷酸(As2O3)对BEL-7402的作用,研究其与硼替佐米联合的化疗效果.方法:As2O3不同浓度及作用时间处理细胞,或与硼替佐米联合作用,倒置显微镜观察细胞形态,MTT法检测增殖,流式AnnexinⅤ-PI双染法检测凋亡,Westernblot及EMSA法检测NF-κB活性.结果:细胞形态及生长抑制率随亚砷酸作用时间及浓度变化而变化,呈时间和浓度依赖性,细胞凋亡率随时间增加而提高(3-48h:5.23%±0.55%,5.24%±0.28%,4.92%±0.91%,4.73%±0.83%,17.54%±1.49%),双药联合作用时,NF-κB活性受到抑制,细胞凋亡率也提升(24h:8.41%±0.78%).结论:As2O3对BEL-7402有明显抑制作用;联合硼替佐米作用,可以增强肿瘤细胞对药物的敏感性,提高肿瘤治疗的效果.AIM： To evaluate the impact of combined treat- ment with arsenous acid and bortezomib on the growth and apoptosis of BEL-7402 cells. METHODS： After BEL-7402 cells were treated with arsenous acid alone or in combination with bortezomib, cell morphology was observed by inverted microscopy; cell proliferation was ana- lyzed by MTT assay; apoptosis was detected by flow cytometry; and NF-κB activity was detected by Western blot and electrophoretic mobility shift assay （EMSA）. RESULTS： Arsenous acid inhibited cell prolif-eration in a time- and concentration-dependent manner, and promoted apoptosis in a time- dependent manner （3-48 h： 5.23% ±0.55%, 5.24% ± 0.28%, 4.92% ± 0.91%, 4.73% ± 0.83%, 17.54% ± 1.49%）. Bortezomib enhanced arsenous acid-me- diated growth inhibition and apoptosis induc- tion （24 h： 8.41% ± 0.78%）, and restrained NF-κB activity. CONCLUSION： Arsenous acid could obviously inhibit growth and promote apoptosis of BEL- 7402 cells. Bortezomib could enhance the sensitivity of BEL-7402 cells to arsenous acid.

关 键 词：核因子ΚB 亚砷酸 硼替佐米 化疗 耐药 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]