丙戊酸钠对肝癌SMMC-7721细胞增殖和细胞周期的影响及机制  

Effect of valproic acid on cell proliferation and cell cycle progression in human hepatoma cell line SMMC-7721

作　　者：钱方方[1] 蒋小猛[2] 徐岷[2] 张尤历[2] 徐萍[1] 王晓燕[2] 吴莺[2] 

机构地区：[1]江苏大学附属医院内分泌科,江苏省镇江市212001 [2]江苏大学附属医院消化科,江苏省镇江市212001

出　　处：《世界华人消化杂志》2012年第1期74-78,共5页World Chinese Journal of Digestology

基　　金：镇江市科研计划基金资助项目;No.SH2009012~~

摘　　要：目的:探讨丙戊酸钠(VPA)对人肝癌SMMC-7721细胞增殖、细胞周期及对p21WAF1/CIP1mRNA表达的影响.方法:实验分为空白对照组、PBS组、VPA0.2mmol/L组、VPA1.0mmol/L组和VPA5.0mmol/L组.不同浓度VPA干预人肝癌SMMC-7721细胞24h、48h和72h,采用MTT法检测细胞存活率,流式细胞仪检测细胞周期;干预72h后,用Real-timePCR法检测VPA干预72h后p21WAF1/CIP1mRNA的表达情况.结果:与空白对照组及PBS组比较,不同浓度的VPA作用24h,48h及72h时组肝癌SMMC-7721细胞增殖均出现了不同程度抑制(请将具体数据列出来P<0.05),随着VPA药物浓度升高,细胞增殖抑制作用逐渐增强,随作用时间延长,抑制程度逐渐增强(P<0.05).随药物浓度升高,G1期细胞比例逐渐增多,S期细胞比例逐渐减少,细胞发生G0/G1期阻滞.VPA干预肝癌SMMC-7721细胞72h后,VPA组p21WAF/CIP1mRNA表达较空白对照组及PBS组表达明显升高(请将具体数据列出来P<0.01).结论:VPA可抑制人肝癌SMMC-7721细胞的增殖,且呈时间及剂量依赖性,并诱导出现G0/G1细胞周期阻滞,同时上调p21WAF1/CIP1mRNA的表达.AIM： To investigate the effect ot valproic acid （VPA） on cell proliferation, cell cycle progres- sion and expression of p21WAF1/CIP1 mRNA in hu- man hepatoma cell line SMMC-7721 in vitro.tometry. The expression of p21WAF1/CIP1 mRNA in SMMC-7721 cells treated with VPA for 72 h was detected by RT-PCR. RESULTS： Compared to the control group and PBS group, treatment with different concentra- tions of VPA for different durations significantly reduced cell growth to a varying extent （all P 〈 0.05）. VPA administration suppressed cell pro- liferation in a time- and dose-dependent man- ner. After treatment with VPA, the percentage of cells in G1 phase increased significantly and that of cells in S phase decreased, suggesting an arrest in G0/G1 phrase. Significant up-regulation of p21WAF1/CIP1 mRNA was observed in SMMC- 7721 cells 72 h after treatment with VPA. CONCLUSION： VPA could significantly sup- press cell proliferation in a time- and dose-de- pendent manner, and result in a cell cycle arrest in G0/G~ phase by inducing elevated expression of p21WAF1/CIP1 mRNA in SMMC-7721 cells.

关 键 词：丙戊酸钠 肝癌 组蛋白去乙酰化酶抑制剂 增殖 细胞周期 

分 类 号：R735.7[医药卫生—肿瘤]