狸猫α-干扰素全基因的克隆及生物信息学分析  

Cloning and bioinformatics analysis of IFN-αgene of wild cat

作　　者：朱前磊[1] 王亚丹[1] 崔保安[1] 陈红英[1] 魏战勇[1] 王子馨[1] 段廷云 

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]漯河出入境检验检疫局,漯河462000

出　　处：《安徽农业大学学报》2012年第1期55-60,共6页Journal of Anhui Agricultural University

基　　金：国家"十五"食品安全重大攻关专项(2001BA804A30-11)资助

摘　　要：根据猫α-干扰素基因核苷酸序列,设计并合成了1对引物,PCR扩增狸猫α-干扰素全基因,并克隆、测序。用DNAStar软件及在线分析方法对克隆的IFN-α基因核苷酸及其推导氨基酸序列进行分析,并与人和其他种动物的IFN-α基因进行同源性和进化分析。结果表明,获得了狸猫α-干扰素全基因序列,其大小为631 bp,编码194个氨基酸的多肽,推导氨基酸序列有1个潜在的N-糖基化位点和7个潜在的O-糖基化位点。IFN-α基因存在种属的差异性,亲缘关系越近,同源性越高。狸猫IFN-α基因的成功克隆为进一步研究猫IFN-α基因表达、生物学活性和应用奠定基础。One pair of primer was designed and synthesized according to the feline interferon alpha gene（IFN-α） nucleic acid sequence.Wild cat IFN-α was amplified by PCR,then cloned and sequenced.The nucleotide sequence of the amplified IFN-α gene and the amino acid sequence deduced from the gene were then analyzed by the DNAstar software and on-line tools,and the similarity and evolution of IFN-α gene were analyzed among wild cat,human and other animals.The results showed the full length sequence of wild cat IFN-α gene was 631 bp,which includes one open-reading frame,encoding 194 amino acid residues,one potential N-glycosalation site and seven O-glcnac sites in deduced amino acid sequence.The results of comparative sequence analysis and phyloge-netic tree analysis indicated there was difference of genus in IFN-α gene,the nearer their relationship,the higher the homology.This study paved the way for future study of biological function and application of feline IFN-α.

关 键 词：狸猫 α-干扰素基因 基因克隆 生物信息学 

分 类 号：Q959.838[生物学—动物学]