荧光光度法结合固相萃取测定亚硝酸盐  

Determination of Nitrite by Spectrofluorimetry with Solid-phase Extraction

作　　者：黄克靖[1] 孙俊永[1] 吴莹莹[1] 

机构地区：[1]信阳师范学院化学化工学院,河南信阳464000

出　　处：《信阳师范学院学报（自然科学版）》2012年第1期99-102,共4页Journal of Xinyang Normal University(Natural Science Edition)

基　　金：河南省高校科技创新人才基金项目(2010HASTIT025);河南省科技创新杰出青年基金项目(104100510020)

摘　　要：以石墨烯为吸附剂制备固相萃取柱,研究影响萃取柱萃取效率的因素.以8-(3’,4’-二氨基苯)-二氟化硼-二吡咯甲烷为荧光探针,在30℃、酸性条件下,与亚硝酸根反应15 min后生成荧光衍生产物.衍生产物经固相萃取后用荧光光度法进行测定,建立了一种荧光光谱法灵敏测定亚硝酸盐的新方法.方法的线性范围为0.2～200 nmol/L.当信噪比等于3时,方法的检出限为0.08 nmol/L.将该方法用于食品中亚硝酸盐的测定,加标回收率为94.0%～110.4%.An efficient solid-phase extraction column was fabricated using graphene as adsorbent.Some factors affecting the extraction efficiency were studied.Fluorescent production was produced after 8-（3’,4’-diaminophenyl）-difluoroboradiaza-s-indacene as fluorescent probe reacted with nitrite in aqueous medium at 30 ℃ for 15 min.A new spectrofluorimetry method was developed for the sensitive determination of nitrite after extracting nitrite derivative.The response was a linear function of concentration in the range of 0.2-200 nmol/L.The detection limit was calculated to be 0.08 nmol/L（RSN=3）.The proposed method was used to determine the content of nitrite in food samples with the recoveries of 94.0%-110.4%.

关 键 词：荧光光度法 石墨烯 亚硝酸盐 食品 

分 类 号：O657.3[理学—分析化学]