In vitro and in vivo Characterization of Homogeneous Chitosan-based Composite Scaffolds  

作　　者：LI Hong ZHOU Changren ZHU Minying TIAN Jinhuan RONG Jianhua 李红;ZHOU Changren;ZHU Minying;TIAN Jinhuan;RONG Jianhua(Department of Materials Science and Engineering,Jinan University;Education Ministry Research Centre of Artif icial Organs&Materials,Jinan University)

机构地区：[1]Department of Materials Science and Engineering,Jinan University [2]Education Ministry Research Centre of Artif icial Organs&Materials,Jinan University

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2012年第1期100-106,共7页武汉理工大学学报（材料科学英文版）

基　　金：the National High Technology Development Program (No. 2007AA091603);the National Natural Science Foundation of China (Nos. 30870612 and 20604010)

摘　　要：With a homogeneous distribution of hydroxyapatite （HAP） crystals in polymer matrix, composite scaffolds chitosan/HAP and chitosanJcollagen/HAP were fabricated in the study. XRD, SEM and EDX were used to characterize their components and structure, in vitro cell culture and in vivo animal tests were used to evaluate their biocompatibility. HAP crystals with rod-like shape embeded in chitosan scaffold, while HAP fine-granules bond with collagen/chitosan scaffold compactly. A homogenous distribution of Ca and P elements both in chitosan/HAP scaffold and chitosan/collagen/HAP scaffold was defined by EDX pattern. The presence of collagen brought a more homogenous distribution of HAP due to its higher ability to induce HAP precipitation. The results of in vitro cell culture showed that the composite＇s biocompatibility was enhanced by the homogenous distribution of HAP. In vivo animal studies showed that the in vivo biodegradation was effectively improved by the addition of HAP and collagen, and was less influenced by the homogeneous distribution of HAP when compared with a concentrated distribution one. The composite scaffolds with a homogeneous HAP distribution would be excellent alternative scaffolds for bone tissue engineering.With a homogeneous distribution of hydroxyapatite （HAP） crystals in polymer matrix, composite scaffolds chitosan/HAP and chitosanJcollagen/HAP were fabricated in the study. XRD, SEM and EDX were used to characterize their components and structure, in vitro cell culture and in vivo animal tests were used to evaluate their biocompatibility. HAP crystals with rod-like shape embeded in chitosan scaffold, while HAP fine-granules bond with collagen/chitosan scaffold compactly. A homogenous distribution of Ca and P elements both in chitosan/HAP scaffold and chitosan/collagen/HAP scaffold was defined by EDX pattern. The presence of collagen brought a more homogenous distribution of HAP due to its higher ability to induce HAP precipitation. The results of in vitro cell culture showed that the composite＇s biocompatibility was enhanced by the homogenous distribution of HAP. In vivo animal studies showed that the in vivo biodegradation was effectively improved by the addition of HAP and collagen, and was less influenced by the homogeneous distribution of HAP when compared with a concentrated distribution one. The composite scaffolds with a homogeneous HAP distribution would be excellent alternative scaffolds for bone tissue engineering.

关 键 词：CHITOSAN HYDROXYAPATITE SCAFFOLD COLLAGEN CHARACTERIZATION 

分 类 号：TB33[一般工业技术—材料科学与工程]