杂交松组织培养技术研究初报  

Preliminary report on the tissue culture techniques of Pinus elliottii×Pinus caribaea

作　　者：方利娟[1] 黄宝灵[2] 苏仕林[1] 

机构地区：[1]百色学院化学与生命科学系,广西百色533000 [2]广西大学林学院,广西南宁530000

出　　处：《广东农业科学》2012年第2期44-46,F0002,共4页Guangdong Agricultural Sciences

基　　金：广西科学研究与技术开发计划项目(桂科攻0235017-6)

摘　　要：以杂交松当年生茎段为外植体,对杂交松进行了组织培养研究。结果表明,最佳诱导培养基为DCR+6-BA 3.0 mg/L+NAA 0.1 mg/L,诱导率为43.3%;最佳增殖培养基为DCR+6-BA 2.5 mg/L+NAA 0.2 mg/L,增殖率为96.7%;最佳生根培养基为1/2DCR+NAA 2.0 mg/L,生根率为53.3%,平均生根数为9.4条/株。Took the current-year-grown stem segments of Pinus eUiottii^Pinus caribaea as explants, we studied the tissue culture of Pinus elliottiiPinus caribaea. The results showed that the best medium for inducing was DCR＋6-BA 3.0 mg/L＋NAA 0.1 mg/L, and the frequency was 43.3%. The best media of propagation was DCR＋6-BA 2.5 mg/L＋NAA 0.2 mg/L, in this instance, the propagation rate was 96.7%. And the best rooting media was I/2DCR＋NAA 2.0 mg/L, in this instance, the rooting rate was 53.3%, the average rooting number was 9.4 in every unit.

关 键 词：杂交松 茎段 组织培养 

分 类 号：Q813.13[生物学—生物工程]