黄鳝性逆转相关基因的克隆和表达  

Clone and expression of a novel sex-related gene in gonads during sex reversal in the rice field eel(Monopterus albus)

作　　者：蒋骄云[1] 冯龙[1] 许世杰[1] 李园园[1] 曲宪成[1] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306

出　　处：《广东农业科学》2012年第2期106-109,共4页Guangdong Agricultural Sciences

摘　　要：运用cDNA末端快速扩增(Rapid-Amplification of cDNA Ends,RACE)技术和半定量RT-PCR技术,克隆了黄鳝(Monopterus albus)性腺差异表达基因(F64)的cDNA全长序列,分析了该基因于各期性腺组织的表达情况。结果表明,F64基因cDNA全长1 721 bp,含有142 bp的5’-UTR、268 bp的3’-UTR、1 311 bp的开放阅读框(ORF),共编码436个氨基酸;在线SignalP分析表明,F64亚基肽链不存在信号肽,为非经典分泌蛋白;同源性分析结果显示该基因无同源性序列,视为新报道的基因;F64在卵巢发育早期不表达,从排卵的Ⅴ期卵巢开始表达,随着卵巢的败育和精巢的发育,表达量明显升高。Using rapid-amplification of cDNA ends and semi-quantitative RT-PCR technique, the full length of the differentially expressed gene （F64） was obtained and the expression in the rice field eel （Monopterus a/bus） was conducted at different gonad developmental stage. The results showed that the full length of F64 was 1 721 base pair （bp） that comprises of a 5＇-UTR of 142 bp, a 3＇ -UTR of 268 bp and an open reading frame （ORF） of 1 311 bp which encoded a 436 amino acid with no signal peptide. Homology analysis suggested that it was a new reporter gene. F64 was not transcription at the early stage of ovary and began to express in ovary at V stage, with the ovary abortive and testis development the transcription level was significantly increased.

关 键 词：黄鳝 性腺 差异表达基因 RT-PCR RACE 

分 类 号：Q786[生物学—分子生物学]