颌骨骨髓基质细胞与牙周膜细胞的生物学特性比较  

A comparative study on the biological characteristics of human periodontal ligament cells and orofacial bone marrow stromal cells

作　　者：王欣[1] 房明[1] 刘敏杰[1] 董广英[1] 王新文[1] 刘玲侠[1] 王勤涛[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2012年第2期66-70,共5页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金资助项目(81070842)

摘　　要：目的:比较颌骨骨髓基质细胞与牙周膜细胞的基本生物学特性。方法:分离颌骨来源骨髓基质细胞和牙周膜细胞,进行改良体外原代培养。倒置显微镜观察细胞生长及克隆形成情况;CCK-8检测细胞生长曲线;免疫荧光染色检测STR0-1表达;成脂诱导后检测脂滴形成,矿化诱导后检测碱性磷酸酶的变化并用RT-PCR检测7、14 d成骨相关基因OCN的表达水平。结果:两种细胞体外培养均呈成纤维样细胞外形,能克隆生长,均具有活跃的增殖能力;两种细胞STR0-1表达阳性;成脂诱导后可见脂滴形成;矿化诱导后骨髓基质细胞的碱性磷酸酶活性较强,而且OCN基因表达较早且较强。结论:颌骨来源骨髓基质细胞具有较强的增殖及成骨分化能力,具有干细胞特性,可能是有较大临床应用潜力的组织工程种子细胞。AIM： To compare the biological characteristics of human orofacial bone marrow stromal cells and periodontal ligament cells in vitro.METHODS：Primary human orofacial bone marrow stromal cells and periodontal ligament cells（PDLCs） were isolated and cultured in vitro.Cell morphology,growth curve,colony formation ratio（CFR）,expression of STRO-1 were analyzed by inverted contrast microscope,cck-8 and immunofluorescence staining.After osteogenic induction,alkaline phosphatase activity and the expression of osteocalcin（OCN） were tested by ELISA and RT-PCR.The adipogenic differentiation potential was identified by oil red O staining.RESULTS： Both BMSCs and PDLCs showed fibroblast-like morphology and clonal growth pattern.They proliferated and expanded rapidly in vitro.Both cells expressed the stem cell marker STRO-1.Alkaline phosphatase activity of BMSCs was higher than that of PDLCs on 7 and 14 days after osteogenic induction.BMSCs expressed OCN gene after 7 days of induction,while PDLCs didn＇t express it.Lipid droplets were observed in both cells after adipogenic induction.CONCLUSION： The orofacial BMSCs show stem cell characteristics and have strong proliferative capability and osteogenic differentiation potential,hopefully for clinical application as tissue engineering seeding cell.

关 键 词：颌骨 人骨髓基质细胞 人牙周膜细胞 潜能 

分 类 号：R780.2[医药卫生—口腔医学]