不同浓度肿瘤坏死因子对人牙周膜细胞增殖和碱性磷酸酶活性的影响  

Effect of tumor necrosis factor-α on proliferation and activity of ALP in human periodontal ligament fibroblasts

作　　者：郝立辉[1] 

机构地区：[1]邢台医学高等专科学校口腔系,河北邢台054000

出　　处：《牙体牙髓牙周病学杂志》2012年第2期74-77,共4页Chinese Journal of Conservative Dentistry

摘　　要：目的:观察不同浓度肿瘤坏死因子(TNF-α)对人牙周膜细胞增殖活性和碱性磷酸酶(ALP)活性的影响。方法:取第4代体外培养人牙周膜细胞(hPDLCs),分别与0、1、5、10、20 ng/mL不同浓度TNF-α共同培养7 d,采用MTT法测定培养后0～7 d各时间点HPDLCs的增殖活力;用实时PCR检测不同浓度TNF-α刺激72 h后细胞周期蛋白(CyclinD1)mRNA的表达;检测ALP活性。结果:与对照组相比,1、5 ng/mL的TNF-α均可促进HPDLCs的增殖和Cyclin mRNA和ALP的表达(P<0.05);10、20 ng/mL浓度可抑制PDLCs的增殖和Cyclin D1 mRNA、ALP的表达(P<0.05)。结论:不同浓度的TNF-α刺激牙周膜细胞后可以影响细胞的增殖和分化。AIM： To study the effect of recombinant human tumor necrosis factor-α（TNF-α） on proliferation and activity of alkaline phosphatase（ALP） of human periodontal ligament cells（HPDLCs） cultivated in vitro.METHODS： The HPDLCs were tested Vimentin positive and Ck（pan） negative.Different dosages（0ng/mL,1ng/mL,5ng/mL,10ng/mL,20ng/mL） of TNF-α were added in the cultured HPDLCs.The proliferation of HPDLCs was examined by MTT assay during a period of 7 d.The mRNA expression of CyclinD1 was tested by real time PCR after 72h.ALP activity was examined under the same condition.RESULTS： Compared with the control group,1ng/mL,5ng/mL of TNF-α significantly elevated the proliferation rate and ALP activity of HPDLCs（P0.05）.However 10ng/mL,20ng/mL of TNF-α significantly reduced the proliferation rate and ALP activity（P0.05）.CONCLUSION： TNF-αmay participate in the remodeling of periodontal tissue by regulating the proliferation and osteogenic differentiation of HPDLCs.

关 键 词：肿瘤坏死因子 牙周膜细胞 增殖 碱性磷酸酶活性 

分 类 号：R780.2[医药卫生—口腔医学]