A small internal antisense RNA (aftsH) of all3642 (ftsH) in Anabaena sp. PCC 7120  

作　　者：GONG YangMin & XU XuDong State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China 

出　　处：《Chinese Science Bulletin》2012年第7期756-761,共6页

基　　金：supported by the National Natural Science Foundation of China (30825003, 30770049)

摘　　要：In bacteria, FtsH proteases are involved in quality maintenance of membrane proteins and cellular regulation at the level of protein stability. Among the 4 predicted ftsH genes in the cyanobacterium Anabaena sp. PCC 7120, namely alr1262, all3642, all4776 and all4936, at least the first two showed reduced transcription in specialized N2-fixing cells called heterocysts. In contrast, the transcription of a small internal antisense RNA (aftsH) of all3642 was up-regulated in heterocysts. Overexpression of the antisense RNA suppressed the expression of all3642, alr1262 and all4776. Control of promoter and modulation by antisense RNA may both contribute to gene regulation in heterocysts of filamentous cyanobacteria.In bacteria, FtsH proteases are involved in quality maintenance of membrane proteins and cellular regulation at the level of pro- tein stability. Among the 4 predicted ftsH genes in the cyanobacterium Anabaena sp. PCC 7120, namely alr1262, a113642, al14776 and al14936, at least the first two showed reduced transcription in specialized N2-fixing cells called heterocysts. In con- trast, the transcription of a small internal antisense RNA （afisH） of ai13642 was up-regulated in heterocysts. Overexpression of the antisense RNA suppressed the expression of a113642, alr1262 and all4776. Control of promoter and modulation by antisense RNA may both contribute to gene regulation in heterocysts of filamentous cyanobacteria.

关 键 词：反义RNA 鱼腥藻 丝状蓝藻 调节蛋白 维修质量 调控基因 蛋白酶 膜蛋白 

分 类 号：Q943[生物学—植物学]