一株产几丁质酶渤海丝状真菌(Alternariatenuissima)的筛选及鉴定  

Screening and identification of chitinase-producing fungus(Alternaria tenuissima) from Bohai Bay

作　　者：刘丹[1] 孟凡欢[1] 李爽[1] 冯欣[1] 孟庆恒[1] 孙建华[1] 

机构地区：[1]天津师范大学生命科学学院,天津市细胞遗传与分子调控重点实验室,天津300387

出　　处：《中国酿造》2012年第2期45-48,共4页China Brewing

基　　金：国家自然科学基金资助项目(30971904)

摘　　要：以渤海湾水域获得的52株丝状海洋真菌为出发菌株,通过平板透明圈法和发酵液酶活性测定,从中筛选出一株产几丁质酶活力较高的海洋真菌mTJ04-1-1,结合形态学方法和18S rDNA-ITS序列分析对其进行鉴定,确定该菌为极细链格孢霉(Alternaria tenuissima mTJ04-1-1)。该菌能在以几丁质为唯一碳源的平板上生长并产生明显透明圈,透明圈与菌落直径的比值(D/d)达到2.81。对发酵液几丁质酶活力分析结果表明,该酶最适反应温度为45℃,最适pH值为6.5,最适条件下酶活性可达3.2U/mL。A chitinase-producing strain of marine-derived fungus roT J04-1-1 was isolated from 52 isolated strains from Bohai-Bay through transparent circle method and chitinase activity determination. The strain was identified as Altemaria tenuissima based on its morphological characteristics and 18S rDNA-ITS sequence. Further experiment proved that mTJ04-1-1 strain grew well on the chitin cultural plates with visible transparent circle. The ratio of transparent circle to colony （D/d） was 2.81. Activity of the chitinase reached 3.2 U/ml under optimal temperature 45℃ and pH value 6.5.

关 键 词：渤海丝状真菌 几丁质酶 筛选 鉴定 18S rDNA.ITS序列 

分 类 号：Q93-331[生物学—微生物学]