交通相关PM2.5对大鼠脾脏凋亡信号的影响  

Effect of traffic-related PM2.5 on apoptotic signals of spleen in rat

作　　者：刘杰静[1] 张志红[1] 赵莹[1] 乔果果[1] 徐建辉[1] 

机构地区：[1]山西医科大学公共卫生学院环境卫生学教研室,山西太原030001

出　　处：《中国预防医学杂志》2012年第2期81-83,共3页Chinese Preventive Medicine

基　　金：国家自然科学基金资助项目(30700655);山西省基础研究计划(青年科技研究基金)项目(2008021053);山西省高校优秀青年学术带头人支持项目(2009)

摘　　要：目的探讨交通相关PM2.5对大鼠脾脏细胞凋亡信号分子的影响,为研究交通相关PM2.5的免疫毒性机制提供实验依据。方法大鼠气管滴注不同剂量[1.5mg/(kg.d),6mg/(kg.d),24mg/(kg.d)]交通相关PM2.5和生理盐水对照,隔天染毒共6次,制备大鼠亚急性免疫毒性模型。染毒结束次日取脾脏组织,提取蛋白和RNA,Western-Blot检测Caspase-3、Caspase-8、Cyt-c、Bcl-2、Fas-l;RT-PCR检测Bid。结果脾脏细胞中Caspase-3/β-actin灰度比值各染毒组(0.322±0.030,0.374±0.024,0.482±0.032)均比对照组(0.254±0.039)增加(P<0.05)。24mg/(kg.d)染毒组Cyt-c/β-actin灰度比值(0.538±0.217)比对照组(0.206±0.046)增加(P<0.05)。各染毒组Bcl-2/β-actin灰度比值(2.440±0.050,1.998±0.094,1.512±0.094)均比对照组(2.921±0.067)低(P<0.05)。各染毒组促凋亡Bid基因表达(0.354±0.026,0.395±0.028,0.492±0.027)比对照组(0.322±0.032)增加(P<0.05)。结论交通相关的PM2.5可从线粒体途径诱导大鼠脾脏出现凋亡。Objective To study the effect of traffic-related PM2.5 on apoptosis of spleen cells in rats and provide experimental basis for the immunotoxicity of traffic-related PM2.5.Methods Rats were exposed to different doses of traffic-related PM2.5（1.5mg/kg.d,6mg/kg.d,24mg/kg.d）or physiological saline by intratracheal instillation.They faced exposure once every other day at a total of six times and the rat model of sub-acute immunotoxicity was made.The following day,while finishing the exposure,protein and RNA of the spleen cells were extracted.Caspase-3,Caspase-8,Cyt-c,Bcl-2 and Fas-l were detected by Western-Blot;Bid was determined by RT-PCR.Results The gray-scale ratios of Caspase-3/β-actin in different PM2.5 groups（0.322±0.030,0.374±0.024,0.482±0.032）significantly increased compared to that in the control group（0.254±0.039）（P0.05）.The gray-scale ratio of Cyt-c/β-actin in high-dose PM2.5 group（0.538±0.217） was significantly higher than that in the control group（0.206±0.046）（P0.05）.The gray-scale ratios of Bcl-2/β-actin in different doses PM2.5 groups（2.440±0.050,1.998±0.094,1.512±0.094） were lower than saline group（2.921±0.067）（P0.05）.The gene expression levels of promotion apoptosis gene,Bid,in different PM2.5 groups（0.354±0.026,0.395±0.028,0.492±0.027）significantly increased compared to that in the control group（0.322±0.032）（P0.05）.Conclusions Traffic-related PM2.5 can induce apoptosis of spleen cells in rat through mitochondrial pathway.

关 键 词：交通尾气 PM2.5 脾脏 凋亡 

分 类 号：R363[医药卫生—病理学]