活性氧生成在8-硝基白杨素诱导人胃癌SGC-7901细胞凋亡中的作用  

Generation of reactive oxygen species in apoptosis of human gastric cancer SGC-7901 cells induced by 5,7-dihydroxy-8-nitrochrysin

作　　者：蒋崇辉 艾小红[2] 杨小红[3] 罗琼[3] 曹建国[3] 

机构地区：[1]衡阳市中心医院药剂科,湖南衡阳421001 [2]南华大学第一附属医院肿瘤内科,湖南衡阳421001 [3]湖南师范大学医学院,长沙410013

出　　处：《山东大学学报（医学版）》2012年第2期34-37,共4页Journal of Shandong University:Health Sciences

基　　金：湖南省自然科学基金项目(03JJY5009);湖南省卫生厅医药科研项目(C20050)

摘　　要：目的研究活性氧生成在8-硝基白杨素(NOC)诱导人胃癌SGC-7901细胞凋亡中的作用。方法体外培养SGC-7901细胞,采用流式细胞术分析细胞凋亡率,ELISA法检测细胞组蛋白/DNA碎片,H2DCFH-DA探针流式细胞仪检测细胞活性氧(ROS)生成。结果 NOC诱导亚G1峰(Sub-G1)细胞百分率增高和细胞组蛋白/DNA碎片增加(P<0.01),促进SGC-7901细胞活性氧生成(P<0.01)。抗氧化剂N-乙酰半胱氨酸(NAC)能有效对抗NOC诱导SGC-7901细胞凋亡作用。结论 NOC诱导SGC-7901细胞凋亡作用与促进活性氧生成相关。Objective To investigate induction of apoptosis and generation of reactive oxygen species （ROS） of human gastric cancer SGC-7901 cells in vitro by 5,7-dihydrox-8-nitrochrysin（NOC）. Methods SGC-7901 cells were cultured in vitro and the percentage of Sub-G1 cell population was detected by PI stained flow cytometry （FCM）. Cell histoneassociated DNA fragments were examined by ELISA assay. The intracellular accumulation of ROS was determined by FCM using the fluorescent probe H2DCFDA. Results NOC significantly increased the percentage of Sub-G1 cell population and historic-associated DNA fragments in SGC-7901 cells in a concentrationependent manner （ P 〈 0.01 ）. NOC also markedly promoted accumulation of ROS （ P 〈 0.01 ）. The pre-incubation of N-acetylcysteine, an antioxidant, effectively antagonized the induction of apoptosis of SGC-7901 cells by NOC. Conclusion NOC induced apoptosis of the SGC-7901 cell line is associated with induction of ROS generation.

关 键 词：胃肿瘤 白杨素 白杨素衍生物 凋亡 活性氧 

分 类 号：R735.2[医药卫生—肿瘤]