FTIR分析共价偶联对抗体蛋白二级结构的影响  

FTIR Analysis of the Impact of Covalent Coupling on the Secondary Structure of Antibody Protein

作　　者：俞思明[1] 彭运平[1,2] 于淑娟[1] 吕欢[1] 

机构地区：[1]华南理工大学轻工与食品学院,广东广州510640 [2]万孚生物技术有限公司,广东广州510640

出　　处：《光谱学与光谱分析》2012年第3期630-634,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(31071564);中央高校基本科研业务费项目(D211016w)资助

摘　　要：用共价偶联的方法制备了免疫胶乳,并利用傅里叶变换红外光谱技术,结合差谱、去卷积、二阶导和曲线拟合等计算机辅助分析方法,研究免疫胶乳中抗体蛋白的二级结构。结果表明,随着pH值的升高以及胶乳浓度的增大,抗体蛋白的有序结构含量增加。由此认为共价偶联会对抗体蛋白的二级结构产生较为显著的影响。The immunolatex was prepared by covalent coupling.FTIR technology combined with substractive spectroscopy,deconvolution,derivation and curve-fitting methods were used to study the structure of the antibody protein on the immunolatex.The result demonstrates that the α-helix strcture of antibody increases with the increase in the pH value and the concentration of latex.So it is concluded that covalent coupling has a great impact on the secondary structure of antibody protein.

关 键 词：免疫胶乳 共价偶联 傅里叶变换红外光谱 抗体蛋白 二级结构 

分 类 号：Q71[生物学—分子生物学]