博来霉素A_5抑制血管生成和抗肿瘤转移作用的实验研究  

Antimetastasis of Lewis lung carcinoma by a novel angiogenesis lnhibitor bleomycin A_5 in a spontaneous metastasis model

作　　者：武利存[1] 孙公甲[1] 张维东[1] 张庆慧[2] 

机构地区：[1]山东省医学科学院基础医学研究所,济南250062 [2]山东医科大学基础医学院,济南250014

出　　处：《中国抗生素杂志》2000年第1期59-61,共3页Chinese Journal of Antibiotics

基　　金：山东省科委资助!文号 961 2 2 670 2

摘　　要：目的:证实博来霉素A5抑制血管生成和抗肿瘤转移作用。方法:将含有0.5μg博来霉素A5的载体置于胚龄7d的鸡胚绒毛尿囊膜(CAM)上,48h后观察抑制血管生成情况。Lewis肺癌皮下接种C57BL6小鼠6d后,在移植瘤周围及其基底部多处局部注射博来霉素A5,每鼠每次0.6mg,每5天1次,共3次,接种后25d处死小鼠,观察皮下移植瘤血管生成抑制情况和对肺转移的抑制作用。结果:博来霉素A5可使载体周围CAM新生血管明显减少甚至消失。Lewis肺癌皮下移植瘤体积小,颜色苍白,毛细血管稀少,很少出血,坏死常见。肺转移抑制率为80%。结论:博来霉素A5能强烈抑制鸡胚CAM和Lewis肺癌皮下移植瘤的血管生成,瘤周局注具有抗转移作用。提示博来霉素A5是一有潜力的血管生长抑制剂。To determine whether bleomycin A 5 can inhibit angiogenesis and cancer metastasis. Methods: 1.The pellet contained 0.5μg bleomycin A 5 was placed onto the 7th day chick embryo chorioallantoic membrane, and antiangiogenesis activity was to be observed 48d later, 2.At the 6th day after Lewis lung carcinoma was inoculated s.c. into the right flank of C 57 BL/6 mice, bleomycin A 5 was topically injected around and beneath the inoculum, 0.6mg per mouse, once every 5 days, and 3 times totally. All mice were sacrificed on the 25th day after tumor implantation. Tumor angiogenesis and lung metastatic foci were examined. Results: 1.A vessel decreased or avascular zone appeared on the chick CAM around the pellet which contained bleomycin A 5. 2. After administration of bleomycin A 5, subcutaneously inoculated tumors were smaller than those of controls, and looked pale with sparse capillaries. Necrosis could be seen commonly. The percent inhibition of lung metastatic foci was 80%. Conclusion: Bleomycin A 5 can inhibit angiogenesis both on the chick CAM assay and in s.c. Lewis lung carcinoma model. Furthermore, it has a markedly antimetastatic effect.

关 键 词：博来霉素A5 LEWIS肺癌 肿瘤转移 

分 类 号：R734.2[医药卫生—肿瘤] R73－361[医药卫生—临床医学]