Early evaluation for treatment efficacy of 5-fluorouracil and hyperthermia on HCT-116 colon cancer cells by fluorine-18-fluorodeoxyglucose uptake  

作　　者：Wang Zhen-xin Zhang Bin Deng Sheng-ming Chen Shao-ji 

机构地区：[1]Suzhou Univ, Affiliated Hosp 1, Dept Nucl Med, Suzhou 215006, Jiangsu, Peoples R China [2]Suzhou Univ, Affiliated Hosp 1, Dept Oncol, Suzhou 215006, Jiangsu, Peoples R China [3]Suzhou Univ, Affiliated Hosp 1, Dept Gen Surg, Suzhou 215006, Jiangsu, Peoples R China

出　　处：《Chinese Medical Journal》2012年第4期657-661,共5页中华医学杂志（英文版）

摘　　要：Backgroud Fluorine-18-fluorodeoxyglucose （18F-FDG） positron emission tomography imaging can be used to assess the treatment efficacy of chemotherapy and prognosis.The aim of this study was to determine the uptake rate of 18F-FDG in colon cancer HCT-116 cells,and to evaluate the treatment efficacy of chemotherapy,hyperthermia and thermo-chemotherapy through the uptake inhibition rate of 18F-FDG.Methods The uptake rate of 18F-FDG in HCT-116 ceils was determined at various experimental conditions.The inhibition rate of cell growth,uptake rate of 18F-FDG and uptake inhibition rate of l8F-FDG in HCT-116 cells treated with 5-fluorouracil （5-FU） at various concentrations were determined.In HCT-116 cells subjected to chemotherapy （5-FU,100 μg/ml）,hyperthermia （43℃,40 minutes） and thermo-chemotherapy for 24 hours,the inhibition rate of cell growth and uptake inhibition rate of 18F-FDG were determined; early apoptosis,the morphology and ultrastructure of HCT-116 cells were examined; and the contents of glucose and lactate dehydrogenase （LDH） in the cell culture medium of HCT-116 cells were determined.One-way analysis of variance （ANOVA） and correlation analyses were conducted by using SPSS 16.0 software.Results The uptake rate of 18F-FDG in HCT-116 cells was （44.25±2.19）%.Under the condition of adding 5-FU at various concentrations for 24 hours,the uptake rate of 18F-FDG was negatively correlated with 5-FU dosage （r=-0.879,P 〈0.01）; the inhibition rate of cell growth revealed a positive correlation with the uptake inhibition rate of 18F-FDG （r=0.831,P 〈0.01）.In HCT-116 cells subjected to hyperthermia,chemotherapy,and thermo-chemotherapy for 24 hours,the uptake inhibition rates of 18F-FDG were （12.94±2.80）%,（28.25±4.59）%,and （21.60±3.68）%,respectively.The early apoptotic rates of HCT-116 cells were （9.80±0.16）%,（19.80±2.40）%,and （15.70±1.80）%,respectively.Moreover,the contents of glucose and LDH in cell culture medium of HCT-116 cells after treatmentsBackgroud Fluorine-18-fluorodeoxyglucose （18F-FDG） positron emission tomography imaging can be used to assess the treatment efficacy of chemotherapy and prognosis.The aim of this study was to determine the uptake rate of 18F-FDG in colon cancer HCT-116 cells,and to evaluate the treatment efficacy of chemotherapy,hyperthermia and thermo-chemotherapy through the uptake inhibition rate of 18F-FDG.Methods The uptake rate of 18F-FDG in HCT-116 ceils was determined at various experimental conditions.The inhibition rate of cell growth,uptake rate of 18F-FDG and uptake inhibition rate of l8F-FDG in HCT-116 cells treated with 5-fluorouracil （5-FU） at various concentrations were determined.In HCT-116 cells subjected to chemotherapy （5-FU,100 μg/ml）,hyperthermia （43℃,40 minutes） and thermo-chemotherapy for 24 hours,the inhibition rate of cell growth and uptake inhibition rate of 18F-FDG were determined; early apoptosis,the morphology and ultrastructure of HCT-116 cells were examined; and the contents of glucose and lactate dehydrogenase （LDH） in the cell culture medium of HCT-116 cells were determined.One-way analysis of variance （ANOVA） and correlation analyses were conducted by using SPSS 16.0 software.Results The uptake rate of 18F-FDG in HCT-116 cells was （44.25±2.19）%.Under the condition of adding 5-FU at various concentrations for 24 hours,the uptake rate of 18F-FDG was negatively correlated with 5-FU dosage （r=-0.879,P 〈0.01）; the inhibition rate of cell growth revealed a positive correlation with the uptake inhibition rate of 18F-FDG （r=0.831,P 〈0.01）.In HCT-116 cells subjected to hyperthermia,chemotherapy,and thermo-chemotherapy for 24 hours,the uptake inhibition rates of 18F-FDG were （12.94±2.80）%,（28.25±4.59）%,and （21.60±3.68）%,respectively.The early apoptotic rates of HCT-116 cells were （9.80±0.16）%,（19.80±2.40）%,and （15.70±1.80）%,respectively.Moreover,the contents of glucose and LDH in cell culture medium of HCT-116 cells after treatments

关 键 词：colonic neoplasms fluorodeoxyglucose F18 5-FLUOROURACIL HYPERTHERMIA treatment outcome 

分 类 号：S858.23[农业科学—临床兽医学] TQ463.54[农业科学—兽医学]