实验性口腔癌的端粒行为异常研究  

A Study on the Association of Telomere Length with the Experimental Carcinogenesis of Oral Cavity

作　　者：高志[1] 毛祖彝[2] 何永文[2] 徐平平[2] 

机构地区：[1]第四军医大学博士后流动站 [2]华西医科大学口腔医学院,610041

出　　处：《华西口腔医学杂志》2000年第1期48-51,共4页West China Journal of Stomatology

基　　金：国家自然科学基金!(编号 3 9670 788)

摘　　要：目的 :探讨DMBA诱导金黄地鼠颊囊癌变过程中端粒长度动态变化。方法 :应用Southern杂交技术分析金黄地鼠颊囊癌变组织端粒DNA序列长度。结果 :金黄地鼠颊囊癌变过程中 ,癌变侧颊囊粘膜组织端粒长度较自身正常对照侧明显缩短 ,尤以诱癌后期缩短速率最快。结论 :端粒长度缩短在癌变早期显著。Objective: The life cycle of cell can be effected by the length oFf telomere. Loss of telomeric DNA during cell proliferation may play a role in chromosome instability, cellular apoptosis and cancer. The purpose of the study is to measure the dynamic changes of telomere length in experimental oral cavity cancer during golden hamster cheek pouch carcinogenesis induced by DMBA. Methods: First, 52 golden hamsters were divided into 2 groups. Four of them weren't done any treatment and were killed after 3 days. The others were covered with DMBA on the surface of cheek pouch on one side in order to induce carcinogenesis, the other side of cheek pouch was treated as the control. Then, the 48 golden hamsters were divided into 4 groups and were killed in 7, 10, 14, 20 weeks. Light microscope was used to observe the pathologic changes. Southern hybridization was used to analysis the dynamic development of the length of telomeric repeat. In different periods, the reduction rate of telomere length was calculated. Results: ①The cheek pouch mucosa uncovered with DMBA had no abnormal pathologic changes, while that covered with DMBA was observed pathologic changes of different degrees. 4 cases of hyperplasia lesions and 8 cases of dysplasia lesions were observed in the 7 week group, 7 cases of dysplasia lesions and 5 cases of mucosa carcinoma in situ in the 10 week group, 5 cases of mucosa carcinoma in situ, 4 cases of squamous cell carcinomas and 3 cases of death in the 14 week group, and 7 cases of squamous cell carcinomas and 5 cases of death in the 20 week group. ②The telomere length of the normal mucosa was reduced with the hamsters age increasing, but the telomere length was significantly shorter than that of the normal control during the mucosa carcinogenesis of cheek pouch. The average shortened length was about 0 225kb (the normal mucosa was 0 17 kb). ③The highest reduction rate (0 3985) was in a later premalignant period. Conclusion: Age is one of the factors that can short telomere length. However, the sp

关 键 词：癌变 端粒长度 颊囊 口腔肿瘤 金黄地鼠 

分 类 号：R739.8[医药卫生—肿瘤]