实验性口腔癌变模型中端粒酶的研究  

Indentification of Telomerase Activity in the Experimental Carcinogenesis of Oral Cavity

作　　者：毛祖彝[1] 高志[2] 徐平平[1] 左晖[1] 何永文[1] 

机构地区：[1]华西医科大学口腔医学院,610041 [2]第四军医大学博士后流动站

出　　处：《华西口腔医学杂志》2000年第1期52-54,57,共4页West China Journal of Stomatology

基　　金：国家自然科学基金!(编号 3 9670 788)

摘　　要：目的 :分析能合成端粒DNA重复序列的端粒酶在DMBA诱导金黄地鼠颊囊癌变动态过程中的动态变化。方法 :采用PCR基础上的端粒DNA重复序列扩增法和ELISA酶免疫技术 ,测定颊囊癌变组织端粒酶动态变化。结果 :在诱癌过程中 ,颊囊粘膜组织端粒酶活性随组织病理学改变向恶性转化而增高 ,与自身对照相比较有显著差异 ,其升高在诱癌后期最明显。结论 :端粒酶激活是肿瘤形成的早期分子标志 ,也是端粒长度稳定的主要因素。Objective:Telomerase is a ribonucleoprotien enzyme which synthesizes telomere DNA repeat sequences and maintain stably telomere length. The activity of telomerase may be necessary for the growth of immortalized cells overcoming cellular senescence. The researches have shown that telomerase activities are associated with most cancers. The purpose of the study is to detect the development of telomerase activity during golden hamster cheek pouch carcinogenesis induced by DMBA. Methods: First, 52 golden hamsters were divided into 2 groups. Four of them weren't done any special treatment and were killed after 3 days. The others were covered with DMBA on the surface of cheek pouch on one side in order to induce carcinogenesis, the other side of cheek pouch was treated as the control. Then, the 48 golden hamsters were divided into 4 groups and were killed in 7, 10, 14 and 20 weeks. The telomeric repeat amplification protocol (TRAP) based on PCR and ELISA was used to analysis the activity of telomerase. Results: ①The expression of telomerase activity existed in normal cheek pouch mucosa of golden hamsters, which meant that telomerase played an important role on controlling cell proliferation. ②The level of telomerase activity gradually increased while hyperplasia and dysplasia was observed in the cheek pouch mucosa covered with DMBA. It reached its top at a later premalignant period and gradually decreased after that. ③There was negative correlation between the degree the telomere length was shortened and the activity of telomerase (r= 0.9654), and there was positive correlation between the reduced rate of telomere length and the activity of telomerase (r=0 9471). Conclusion: The activity of telomerase is one of important factors that can effect the stability of telomere length and plays a crucial role in the progression of oral cancer. So the activity of telomerase is an early molecular marker of carcinogenesis.

关 键 词：癌变 端粒酶 活性 颊囊 口腔肿瘤 

分 类 号：R739.8[医药卫生—肿瘤]