皮肤病理性瘢痕和瘢痕癌中Ras基因家族12/13位密码子突变的检测  

Mutation of ras gene family codons 12 and 13 in skin pathological scar and scar carcinoma tissues

作　　者：胡成久[1] 郭瑞珍[1] 王燕[2] 

机构地区：[1]遵义医学院珠海校区病理学教研室,广东省珠海市519041 [2]遵义医学院珠海校区分子生物学教研室,广东省珠海市519041

出　　处：《实用医学杂志》2012年第4期576-578,共3页The Journal of Practical Medicine

基　　金：贵州省攻关项目(编号:2010-3080)

摘　　要：目的:探讨皮肤病理性瘢痕和瘢痕癌中Ras基因家族(H-ras/N-ras/K-ras)12/13位密码子的突变情况。方法:从14例皮肤病理性瘢痕,14例瘢痕癌石蜡包埋组织中提取DNA,进行PCR扩增及测序,分析H-ras、N-ras、K-ras基因第12、13位密码子突变情况。结果:28个样本均成功提取DNA,扩增出基因片段,经测序均未发现H-ras、N-ras、K-ras基因第12、13位密码子的点突变。结论:皮肤病理性瘢痕和瘢痕癌中Ras基因家族的突变位点有待进一步探讨。Objective To explore the mutation of Ras gene family（H-ras /N-ras /K-ras） in skin pathological scar and scar carcinoma tissues.Methods The DNA was extracted from the choosen samples fixed in 10% formalin and Paraffiembedded（including 14 cases skin pathological scar tissues and 14 cases skin scar carcinoma tissues）,H-ras,N-ras and K-ras mutations in codons 12 and 13 were analyzed by PCR and sequencing.Results DNA was extracted from the choosen 28 samples.By PCR,the 28 samples were succeed to enlarge the gene piece.Without gene mutation in codons 12 and 13 of H-ras,N-ras and K-ras genes were examined by sequencing.Conclusions The mutation situs of Ras family is to be further explored in skin scar and scar carcinoma.

关 键 词：突变 RAS基因 皮肤病理性瘢痕 瘢痕癌 

分 类 号：R622[医药卫生—整形外科]