食用菌中多种氨基甲酸酯类农药残留的超高效液相色谱-串联质谱法测定研究  

Study on UPLC-MS/MS Method for Determination of Carbamate Pesticide Residue in Mushrooms

作　　者：赵颖[1] 蒋施[1] 金雁[1] 姚家彪[1] 刘瑜[1] 徐宜宏[1] 钟钰[1] 吕红英[1] 李梅[1] 

机构地区：[1]沈阳出入境检验检疫局综合技术中心,沈阳10016

出　　处：《化学通报》2012年第2期150-154,共5页Chemistry

基　　金："十一.五国家科技支撑计划"课题"农药与兽药残留确证检测技术研究"项目(2006BAK02A08-005)资助

摘　　要：建立了同时测定食用菌中19种氨基甲酸酯类农药(NMCs)残留的超高效液相色谱-串联质谱法(UPLC-MS/MS)。通过丙酮和乙腈(85∶15,V/V)提取,GPC法和PSA固相萃取柱两种方法净化,UPLC-MS/MS同时测定蘑菇、香菇、木耳和牛肝菌中19种氨基甲酸酯类农药,同位素稀释法定量。以GPC法净化,回收率为74.0%～93.2%;以PSA固相萃取柱净化,回收率为70.2%～91.8%,两种方法的检出限均为0.01mg/kg。各种NMCs在确定的添加范围内线性关系良好,线性相关系数R均大于0.99。所建立的检测方法实用、准确、灵敏度高。Methods to simultaneously determine 19 carbamate pesticides residues in mushrooms by liquid chromatography tandem mass spectrometry （UPLC-MS/MS）were established. The mushroom samples were extracted with acetone/acetonitrile （85： 15, V/V） , purified by gel permeation chromatography（GPC） or solid phase extraction （SPE） and detected by UPLC-MS/MS with isotope internal standard method. Results showed that the recoveries ranged from 74. 0% to 93.2% with the cleaning method of GPC and 70. 2% to 91.8% with the cleaning method of PSA solid phase extraction. The limits of detection （LOD） was 0.01mg/kg. The method showed a good linear relationship with the correlation coefficients over 0. 99. The established method is applied, accurate and high sensitive.

关 键 词：食用菌 氨基甲酸酯 超高效液相色谱-串联质谱 GPC PSA 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.63[轻工技术与工程—食品科学与工程]