不同强度20Hz重复经颅磁刺激对脑梗死大鼠神经行为学及胶质纤维酸性蛋白的影响  

Effects of 20 Hz repetitive transcranial magnetic stimulation with different intensities on neurobehavior and glial fibriilary acidic protein in rats with cerebral infarction

作　　者：马玉娟[1] 黄杰[1] 方征宇[1] 随燕芳[1] 种玉飞[1] 尤春景[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院康复医学科,武汉430030

出　　处：《中华物理医学与康复杂志》2012年第2期85-88,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：基金项目：国家自然科学基金项目（30801220）,湖北省自然科学基金项目（2008CDB383）

摘　　要：目的观察不同强度20Hz重复经颅磁刺激（rTMS）对脑梗死大鼠神经行为学及脑缺血半暗带区胶质纤维酸性蛋白（GFAP）表达的影响，并探讨rTMS的治疗机制。方法采用随机数字表法将43只SD大鼠分为正常组、模型组、假刺激组及rTMS组，rTMS组又按照磁刺激强度不同细分为80％运动阈值（MT）组、100％MT组和120％MT组。除正常组外，余各组大鼠均制成右侧大脑中动脉栓塞（MCAO）模型。各rTMS亚组于制模24h后给予20Hz相应强度磁刺激，假刺激组则给予假磁刺激，模型组制模后未给予特殊处理。于制模后第1、8、15天时观察各组大鼠神经行为学变化情况，于制模后第15天时采用免疫组化法观察各组大鼠缺血半暗带区GFAP表达情况。结果制模后第15天时100％MT组神经功能缺损评分、平衡木评分及铁丝抓握评分均较制模后第1天时明显改善，其他各组仅有上述2种神经行为学评分较制模后第1天时明显改善（P〈0．05）；免疫组化检测显示各rTMS亚组缺血半暗带区GFAP表达均较模型组显著减少（P〈0．05）。结论低中强度（尤其是100％MT强度）20HzrTMS刺激能显著促进急性期脑梗死大鼠神经功能改善，其治疗机制可能与减少脑梗死灶周边GFAP表达有关。Objective To investigate the effects of 20 Hz repetitive transcranial magnetic stimulation （rTMS） with different intensities on neurobehavior and expression of glial fibrillary acidic protein （GFAP） in ischemie penumbra of rats with cerebral infarction, so as to explore the probable mechanism. Methods Forty-three rats were randomly divided into a blank control group（n =7） , a mode] control group（ n =7） , a sham stimulation control group（n =8） and a rTMS group （n =21） ; the rTMS group was further subdivided into 3 subgroups： 80% NIT sub- group, 100% MT subgroup and 120% MT subgroup, with 7 rats in each subgroup. The cerebral infarction model was established by right middle cerebral artery occlusion （MCAO） in each group except the blank control group. The 3 rTMS subgroups were given 14 successive blocks of 20 Hz rTMS with corresponding intensity. The sham stimulation control group received sham treatment （without any output）. The model control group was given no stimulation, and the blank control group did not receive any special treatment. Functional assessments were performed at 3 different time points. After 14-day treatment, the expression of GFAP proteins in ischemic penumbra were detected by immu- nohistoehemistry technique. Results Functional outcome reflected from 3 behavioral tests in 100% MT subgroup after 14-day stimulation was better than 1 day after operation, while in the other rTMS subgroups functional outcomes were just better in 2 behavioral tests. The expressions of GFAP in 3 rTMS subgroups were all less than that in model control group. Conclusions The 20 Hz rTMS with 80% MT and 100% MT might be safe and effective to improve the functional outcome in rats with acute cerebral infarction, especially 100% MT. Decrease of expression of GFAP in ischemic penumbra might be one of the mechanisms of beneficial effects of rTMS in ischemia brain injury.

关 键 词：脑梗死 重复经颅磁刺激 神经功能评估 胶质纤维酸性蛋白 

分 类 号：R743.33[医药卫生—神经病学与精神病学]