大豆异黄酮和皂甙对肝癌前病变大鼠血清标志酶及抗氧化活性的影响  

Effect of Soybean Isoflavones and Saponins on Serum Marker Enzymes and Antioxidative Activities of Rat with Hepatic Preneoplasia

作　　者：许惠仙[1] 汪霞[1] 金延华[1] 尹学哲[1] 

机构地区：[1]延边大学附属医院,吉林延吉133000

出　　处：《大豆科学》2012年第1期124-126,共3页Soybean Science

基　　金：吉林省科技发展计划资助项目(200705428);国家自然科学基金资助项目(30360113)

摘　　要：采用改良Solt-Faber法建立大鼠肝癌前病变模型,以大豆异黄酮和皂甙饲喂大鼠42 d后,ELISA法测定血清肿瘤坏死因子-α(TNF-α),分光光度法测定γ-谷酰胺转肽酶(γ-GT)、丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活性及丙二醛(MDA)和一氧化氮(NO)含量。结果表明:大豆异黄酮和皂甙降低肝癌前病变大鼠血清γ-GT、ALT、AST活性,升高血清SOD、CAT、GSH-PX活性和降低MDA以及NO水平,但对血清TNF-α水平没有显著影响。表明大豆异黄酮和皂甙具有明显的抗化学致癌作用,其作用机制可能与增高抗氧化活性有关。The hepatic preneoplastic lesions were induced by the modified protoco1 of Solt and Farber.Rats were administered with soybean isoflavones and saponins for 42 days,then the tumor necrosis factor-α（TNF-α）was determined with ELISA method.The activities of γ-glutamyl transpeptidase（γ-GT）,alanine trasaminase（ALT）,aspartate aminotransferdse（AST）,superoxide dismutase（SOD）,catalase（CAT）,glutathione peroxidase（GSH-PX）,as well as the contents of malondialdehyde（MDA）and nitric oxide（NO）were detected by the spectrometric method.Soybean isoflavones and saponins intake reduced the activities of serum γ-GT,ALT and AST,increased the serum SOD,CAT,GSH-PX activities,and decreased the serum MDA and NO levels in rats with hepatic preneoplastic lesions,however,no significant effect on serum TNF-α level was detected.It is suggested that soybean isoflavones and saponins exhibited an inhibitory effect on formation of hepatic preneoplastic lesions in rats,probably via its antioxdative activity.

关 键 词：大豆 异黄酮 皂甙 肝 癌前病变 抗氧化 

分 类 号：R735.7[医药卫生—肿瘤]