鸡源性多重耐药沙门氏菌 类整合子与耐药基因研究  

Detection of Integron Ⅰ and Drug Resistance Genes of Multidrug-resistance Salmonella Isolated from Chickens

作　　者：赵玉林[1] 刘芳萍[1] 李昌文[2] 刘立新[1] 李睿[1] 罗鹏志[1] 卢斯亮[1] 张秀英[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001

出　　处：《中国家禽》2012年第4期29-32,共4页China Poultry

基　　金：黑龙江省教育厅自然科学面上项目(11541030)

摘　　要：本试验旨在了解鸡源性沙门氏菌分离株多重耐药与Ⅰ类整合子及耐药基因的携带关系。采用K-B纸片法对29株鸡源性沙门氏菌分离菌株进行10种抗菌药物敏感试验;应用PCR技术对分离菌株进行Ⅰ类整合子及耐药基因检测。29株分离株中有13株对2种以上抗菌药物耐药,属于多重耐药株,氨苄西林-四环素-头孢唑啉-复合磺胺是主要多重耐药谱;13株多重耐药菌中有8株携带Ⅰ类整合子,blatem-1、tetA和tetB基因检出最高。结果表明沙门氏菌多重耐药性与整合子的携带之间关系密切,耐药表型测定结果与耐药基因检测结果基本一致。To determine the relationship between integron I and drug resistance genes multidrug-resistance Salmonella isolated from chickens,the sensitivity tests of 29 strains to 10 antibacterials were performed by Kirby-Bauer method,the integron I and resistance genes were detected by PCR. There were 13 strains were multidrug resistance strains which showed resistance to more than two kinds of antibacterials. Ampicillin-Tetracycline-Cefazolin Compound Sulfadiazine was the main muhidrug resistance spectrum,and there were 8 multidrug resistance strains carried integron I ,and blatme-l,tetA and tetB were the predominant genes. These results suggested that multidrug-resistance Salmonella had a close correlation with carried integron, the detection of resistance phenotype agreed with the detection of resistance genes.

关 键 词：鸡 多重耐药 沙门氏菌 Ⅰ类整合子 耐药基因 

分 类 号：S852.612[农业科学—基础兽医学]