胃癌患者外周血中鸟苷酸环化酶C信使RNA的表达及其临床意义  

Expression of GC-C mRNA in peripheral blood mononuclear cells and its clinical roles in determining state of gastric cancer

作　　者：张健锋[1] 薛世民[1,3] 张弘[1] 孟海[1] 朱惠君[2] 毛振彪[1] 

机构地区：[1]南通大学附属医院消化内科,江苏省南通市226001 [2]南通大学附属医院病理科,江苏省南通市226001 [3]陕西省榆林市第二医院呼吸内科

出　　处：《中华医学杂志》2012年第6期371-375,共5页National Medical Journal of China

摘　　要：目的探讨胃癌患者外周血中鸟苷酸环化酶C信使RNA（GC-CmRNA）的表达及其与外周血微转移及病情判断的关系。方法实时荧光定量PCR（RFQ-PCR）检测2009年11月至2010年8月在南通大学附属医院住院的60例胃癌患者和门诊21例异型增生、15例肠上皮化生患者及20名健康体检者外周血中GC．CmRNA的表达，同时检测在这60例胃癌患者手术切除胃癌组织中GC-CmRNA的表达。对数据进行Wilcoxon秩和检验、x。检验或Fisher确切概率法分析，外周血和胃癌组织中GC-CmRNA表达的相关性分析采用Spearman秩相关分析。结果在健康体检者外周血中未检测到GC-CmRNA表达，肠上皮化生和异型增生患者外周血中分别检测到2例和3例阳性，胃癌患者外周血中检测到GC-CmRNA高表达，其表达阳性率为48．3％（29／60），与健康体检者（0）、肠上皮化生（9．5％，2／21）和异型增生（20．0％，3／15）比较，差异均有统计学意义（均P〈0．05），且其表达与Lauren分型、临床分期、分化程度、浸润深度和淋巴结转移等因素均有关（均P〈0．05）。相关性分析显示外周血和胃癌组织中GC-CmRNA的表达呈正相关（r=0．4009，P=0．0015）。结论胃癌患者外周血中存在GC-CmRNA表达，且其表达与患者病情进展有关，有望成为胃癌预后判断新的标志物。Objective To detect the expression of GC-C mRNA in peripheral blood of gastric carcinoma patients and determine its potential candidacy as a molecular biological marker for predicting the micrometastasis and determining the status of gastric cancer. Methods GC-C mRNA from peripheral blood samples of gastric carcinoma （ n = 60 ）, displastia （ n = 21 ）, intestinal metaplasia （ n = 15 ） and healthy cases （n = 20 ） from November 2009 to August 2010 at Affiliated Hospital of Nantong University were assessed by real-time fluorescent quantitative PCR （RFQ-PCR）. Results The expressions of GC-C mRNA in peripheral blood from patients with dysplasia, intestinal metaplasia and healthy controls were absent or very low. And a high level of GC-C mRNA was detected in the patients with gastric carcinoma than those with displastia and intestinal metaplasia, and the positive rate were 48.3% （29/60）, 9.5% （2/21）, 20.0% （3/15）, respectively （ all P 〈 0.05 ）. The levels of GC-C mRNA were significantly correlated with Lauren typing, clinical stage, tumor differentiation degree, depth of invasion and lymph node metastasis （ all P 〈 0. 05 ）. The GC-C mRNA expressions were positively correlated in peripheral blood and gastric carcinomas tissues （ r = 0. 4009, P = 0. 0015 ）. Conclusions The over-expression of GC-C mRNA is found in peripheral blood from gastric carcinoma patients. Due to its close correlation with clinical stage and lymph node metastasis, it may become a potential prognostic marker of gastric carcinoma.

关 键 词：胃肿瘤 鸟苷酸环化酶 预后 

分 类 号：R734.2[医药卫生—肿瘤]