新型烟酰胺腺嘌呤二核苷酸(NAD)类似物的合成及其辅酶活性  

Synthesis of Novel Nicotinamide Adenine Dinucleotide (NAD) Analogs and Their Coenzyme Activities

作　　者：侯淑华[1,2] 刘武军[1] 赵宗保[1] 

机构地区：[1]中国科学院大连化学物理研究所生物技术部,大连116023 [2]中国科学院研究生院,北京100049

出　　处：《有机化学》2012年第2期349-353,共5页Chinese Journal of Organic Chemistry

摘　　要：尝试4种形成焦磷酸键的方法合成了5种结构新颖的新型烟酰胺腺嘌呤二核苷酸(NAD)类似物.初步考察了类似物的生物活性,发现苹果酸酶和醇脱氢酶以类似物3b和3d为辅酶时,活性只有以NAD为辅酶时的13%～30%;而以类似物3a,3c和3e为辅酶时,这些酶的活性均极低.Four methods were tested to synthesize nicotinamide adenine dinucleotide（NAD） analogs,and 5 NAD analogs were prepared.Assays of these NAD analogs indicated that malic enzyme and alcohol dehydrogenase had about 13%～30% enzymatic activity in the presence of analog 3b or 3d,whereas significantly lower activities were observed in the presence of analog 3a,3c or 3e.

关 键 词：NAD类似物 焦磷酸 生物活性 醇脱氢酶 

分 类 号：Q524[生物学—生物化学]