蛇毒精氨酸酯酶Agkihpin抑制人肝癌SMMC-7721细胞株MRP1表达  

The Inhibition of Venin Arginine Esterase Agkihpin in the Expression of MRP1 in the SMMC-7721 Cell Strain

作　　者：胡启平[1] 许淑茹[2] 黄程新[1] 马军[1] 方玲[1] 袁志刚[1] 

机构地区：[1]广西医科大学细胞生物学与遗传学教研室南宁市530021 [2]漳州卫生职业学院

出　　处：《中国肿瘤临床》2012年第4期181-184,共4页Chinese Journal of Clinical Oncology

基　　金：广西自然科学基金(编号:桂科青0728059);广西大型仪器协作共用网(编号:00-2008-113)资助~~

摘　　要：目的:探讨蛇毒精氨酸酯酶Agkihpin对人肝癌细胞株SMMC-7721中多药耐药相关蛋白1(multidrug resistance associated protein 1,MRP1)表达的影响,并阐明Agkihpin抑制人肝癌SMMC-7721细胞活力的机制。方法:采用不同浓度的Agkihpin处理SMMC-7721细胞72 h后,应用免疫细胞化学、Western blot和RT-PCR等方法检测MRP1在SMMC-7721细胞中的转录和表达。结果:不同浓度Agkihpin作用SMMC-7721细胞72 h后MRP1表达均降低,显示Agkihpin可显著下调SMMC-7721细胞中MRP1转录和表达(P<0.05),并呈现出一定的浓度依赖效应。结论:Agkibpin能抑制人低分化肝癌细胞株SMMC-7721中MRP1的表达,并呈一定程度的浓度依赖效应,提示Agkihpin在一定程度上可用于提高肝癌细胞对化疗药物的敏感性。Objective： To explore the effects of Agkihpin on the expression ofmultidrug resistance-associated protein 1 （ MRP1 ） in the SMMC-7721 cell line and to describe the mechanism of SMMC-7721 inhibition by Agkihpin. Methods： The cultured cells were treated with different concentrations of Agkihpin for 72 h. The transcription and expression levels of MRP 1 in SMMC-7721 were assayed using immunocytochemistry, Western blot, and reverse transcription-polymerase chain reaction. Results： Compared with the control group, both the transcription and the expression levels of MRP 1 in SMMC-7721 were significantly reduced after treatment with different concentrations ofAgldhpin （ P 〈 0.05 ） in a concentration-dependent manner. Conclusion： Agkihpin can inhibit the transcription and expression of MRP1 in SMMC-7721 cells, which is likely responsible for the inhibition of cellular vitality and, to some extent, the enhancement of the sensitivity of the hepatocellular carcinoma cells to chemotherapeutics.

关 键 词：多药耐药相关蛋白1 精氨酸酯酶 肝癌细胞 

分 类 号：R735.7[医药卫生—肿瘤]