低氧无血清对乳鼠心肌细胞皮肤桥蛋白表达的影响  

Effect of hypoxia and serum deprivation on the expression of dermatopontin in cultured neonatal rat cardiomyocytes

作　　者：刘晓艳[1] 张秀芳[1] 魏英杰[1] 史强[1] 崔传珏[1] 白媛媛[1] 

机构地区：[1]北京协和医学院中国医学科学院阜外心血管病医院心血管转化医学国家重点实验室,北京100037

出　　处：《基础医学与临床》2012年第3期257-260,共4页Basic and Clinical Medicine

基　　金：高等学校博士学科点专项科研基金(20091106110018);国家高技术研究发展计划(863计划)(2006AA02Z4B8)

摘　　要：目的在体外用低氧无血清条件模拟缺血心肌微环境,探讨心肌细胞皮肤桥蛋白(DPT)表达的变化。方法以低氧无血清处理乳鼠心肌细胞0、6、12和24 h,用实时反转录聚合酶链式反应检测DPT mRNA的表达变化,用Western blot检测DPT的蛋白表达变化。用酶联免疫吸附实验(ELISA)证实低氧无血清处理乳鼠心肌细胞24 hDPT蛋白的表达变化。结果与0 h组相比,低氧无血清处理心肌细胞6、12、24 h DPT的mRNA和蛋白水平均显著升高(P<0.05)。用ELISA法也检测到低氧无血清处理心肌细胞24 h DPT的表达水平高于正常对照组(P<0.05)。结论低氧无血清可促进乳鼠心肌细胞DPT的表达,DPT可能在缺血缺氧引起的心室重构中发挥一定的作用。Objective To investigate the expression of dermatopontin（DPT） induced by hypoxia and serum deprivation（H/SD） which simulated the microenvironment of ischemic myocardium in cultured neonatal rat cardiomyocytes in vitro.Methods Cultured neonatal rat cardiomyocytes were disposed to H/SD for 0,6,12 and 24 h.The mRNA and protein expression of DPT was determined by real time RT-PCR and western blot,respectively.Using enzyme-linked immunosorbent assay（ELISA） to determine the different expression of DPT between normal cultured cardiomyocytes and cardiomyocytes disposed to H/SD for 24 h.Results Compared the data of 0 h,the mRNA and protein level of DPT were significantly raised in cultured cardiomyocytes which were disposed to H/SD for 6,12 and 24 h（P0.05）.It was confirmed by the method of ELISA that compared with control group,the expression of DPT was higher in cardiomyocytes which were disposed to H/SD for 24 h.ConclusionsThe DPT expression in cultured neonatal rat cardiomyocytes was promoted by H/SD in vitro,which suggested that DPT may be invoved in the ventricular remolding process caused by hypoxia and ischemic in vivo.

关 键 词：皮肤桥蛋白 低氧无血清 心肌细胞 

分 类 号：R363[医药卫生—病理学]