不同脂肪含量的高脂纯化配方饲料对大、小鼠代谢综合征发生的影响  

Effect of diets with different fat content on metabolic syndrome of rats and mice

作　　者：周云枫[1,2] 李沙[1,2] 苏文[1,2] 黄世铮[1,2] 蒲丹[1,2] 管又飞[1,2] 

机构地区：[1]北京大学糖尿病中心肥胖及代谢病研究中心,北京100191 [2]北京大学医学部基础医学院生理学与病理生理学系,北京100191

出　　处：《基础医学与临床》2012年第3期273-277,共5页Basic and Clinical Medicine

基　　金：科技部973项目(2012CB517504);国家自然科学基金(81100611);高等学校博士学科点专项科研基金(20110001120020)

摘　　要：目的探讨不同脂肪含量的高脂纯化配方饲料对大、小鼠体重、血糖、血胰岛素、血脂、脂肪肝及白蛋白尿的影响。方法雄性SD大鼠及C57BL/6小鼠随机分为MD10%、MD45%和MD60%脂肪含量饲料组,喂养3个月,检测动物体重、血糖、血胰岛素水平、血脂和蛋白尿水平,以及主要脏器重量及肝脏脂质含量。结果与正常组(MD10%脂肪含量)相比,高脂组(MD45%和MD60%脂肪含量)动物体重显著增加,出现明显的糖耐量异常、胰岛素抵抗和血脂水平升高,同时伴有肝脏脂质含量和蛋白尿水平的增加,并且以MD45%高脂组体重增加及胰岛素抵抗更加显著。结论MD45%和MD60%高脂配方饲料均可在大、小鼠较好地诱导代谢综合征的发生,而且前者优于后者。Objective To examine potential effect of diets with different fat content on body weight,blood glucose and insulin levels,serum total cholesterol（Tch） and triglycerides（TG） concentrations,fatty liver and daily urinary albumin excretion in rats and mice.Methods Males Sprague Dawley（SD） rats and C57BL/6 mice were randomly divided into three groups and fed on diets with 10% fat content（MD10% fat）,45% fat content（MD45% fat）,and 60% fat content（MD60% fat） for 12 weeks,respectively.Body and organ weight,blood glucose and insulin levels,and serum Tch and TG concentrations,liver Tch and TG contents and daily urinary albumin excretion were measured.Results Compared with the controls（MD10% fat）,the animals fed with high-fat diets（MD45%f at and MD60% fat） showed a significant increase in body weight,blood glucose and insulin levels,glucose intolerance,serum Tch and TG concentrations,hepatic Tch and TG contents and albuminuria levels.Furthermore,body weight gain and insulin resistance were more profound in animals fed with the MD45% fat diet.Conclusions Both high-fat diets（MD45% fat and MD60% fat） can produce most phenotypes of metabolic syndromein SD rats and C57BL/6 mice,with the former being more effective.

关 键 词：高脂饲料 胰岛素抵抗 肥胖 代谢综合征 

分 类 号：R589[医药卫生—内分泌]