RP-HPLC法测定豆制品中大豆异黄酮组分含量  

Determination of Flavonoids in Bean Products by RP-HPLC

作　　者：李艳[1] 李沁媛[1] 谭涛[1] 张建红[1] 衡婷[1] 康菲[1] 边清泉[1] 

机构地区：[1]绵阳师范学院化学与化学工程学院,四川绵阳621000

出　　处：《食品与发酵科技》2012年第1期80-82,102,共4页Food and Fermentation Science & Technology

基　　金：绵阳市科技局自然科学研究重点项目(10S002-2);绵阳师范学院科学研究项目(hx2011-2012-08)

摘　　要：采用高效液相色谱法测定豆类及豆制品中大豆异黄酮的含量。采用LC 20AT高效液相色谱系统,以甲醇∶水=5.5∶4.5(v/v)为流动相,检测波长为260nm,流速为0.8mL.min-1。待测组分与其他组分分离度良好,结果表明染料木苷、大豆黄素、染料木素线性范围分别为11.47-172μg.mL-1(r=0.9998),5.33-80μg.mL-1(r=0.9999),8.26-124μg.mL-1(r=0.9998),平均回收率分别为98.9%,98.7%,99.4%,相对标准偏差分别为0.3%,1.8%,2.9%。本试验结果对不同人群,根据各自需要选择豆制品具有一定指导作用。The content of soybean isoflavones in different bean products was determinated by HPLC. LC 20AT was used HPLC system： methanol：water=5.5：4.5 （v/v） was as mobile phase; Detection wavelength was 260nm and flow rate was 0.8mL.min^-1. Good separation of the conponents determined from other coexisiting concentration. The results showed that the genistin, daidzein and genistein perform a good linear relationship in the range of 11.47-172.0μg .mL-1 （r=0.9998）, 5.33-80.01xg.mL-l （r=0.9999） and 8.26-124.0μg-mL^-1 （r=0.9998）. The average recovery of 98.9% for genistin, 98.7% for daidzein and 99.4% for genistein with RSD＇s 0.3%, 1.8% and 2.9% respectively. The test resuits provided some guidance to diffident people to selecte soybean products according to their different needs.

关 键 词：豆制品 HPLC 黄酮类 含量 

分 类 号：TS207.3[轻工技术与工程—食品科学]