PI3K/AKT信号通路在急性白血病中调控机制的初步研究  

Regulatory Mechanisms of PI3K/AKT Signaling Pathway in Acute Leukemia

作　　者：王伟丽 张英弛 曾慧敏 华春兰 魏蔚 许静 竺晓凡 程涛 袁卫平 

机构地区：[1]中国医学科学院、北京协和医学院,血液学研究所、血液病医院,实验血液学国家重点实验室,天津300020

出　　处：《中国实验血液学杂志》2012年第1期18-21,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目(编号81070390),国家自然科学基金资助项目(编号81090414);国家973项目(编号2011CB964801)

摘　　要：本研究探讨PI3K/AKT通路中的PTEN、CCND1、mTOR、RICTOR、FOXO1基因在急性髓系白血病(AML)、急性淋巴细胞白血病(ALL)患者中与正常人中表达的差异,以期查明PI3K/AKT通路在白血病中是否存在通路失调。随机收集16例骨髓标本,其中白血病12例(AML 6例,ALL 6例),正常骨髓标本4例。用实时定量RT-PCR方法检测PI3K/AKT通路中的PTEN、CCND1、mTOR、RICTOR、FOXO1基因的表达变化;以管家基因GAPDH为内参,按2-△△Ct法计算目的基因相对表达量。结果表明:PTEN、mTOR、RICTOR在AML、ALL中总体呈低表达趋势,PTEN在12例标本中有10例低表达,mTOR在12例标本中9例低表达,RICTOR在12例标本中7例低表达;FOXO1,CCND1在AML、ALL中则呈高表达趋势,FOXO1在12例标本中有9例高表达,CCND1在12例标本中7例高表达。结论:PI3K/AKT信号通路基因在白血病细胞中被激活。This study was aimed to analyze the expression profiles of PI3K/AKT signaling pathway genes from bone marrow samples of AML and ALL patients and normal samples. AML, ALL and normal bone marrow samples were col- lected from 6 AML, 6 ALL patients and 4 normal persons. The expression of PI3K/AKT signaling pathway genes inclu- cling PTEN, CCND1, mTOR, RICTOR, FOX01 were detected by real-time fluorescent quantification RT-PCR while GAPDH gene expression was used as an internal reference. The relative gene expression level was calculated by the method of the 2- zxzxct. The results showed that the gene expression profiles were different between normal and leukemic groups. PTEN, mTOR and RICTOR expression levels were down-regulated, while FOX01 and CCND1 levels were up- regulated in AML and ALL. PTEN was down-regulated in 10 out of the 12 samples; mTOR was down-regulated in 9 out of the 12 samples; R1CTOR was down-regulated in 7 out of the 12 samples; FOX01 was up-regulated in 9 out of the 12 samples and CCND1 was up-regulated in 7 out of the 12 samples. It is concluded that PI3K/AKT signal pathway is acti- vated in both AML and ALL leukemic cells.

关 键 词：PI3K/AKT AML ALL 白血病 

分 类 号：R733.71[医药卫生—肿瘤]