香菇多糖对抗模拟微重力环境培养的淋巴细胞免疫功能抑制的实验研究  

Effect of Lentinan against Immunosuppression of Lymphocytes Cultured in Simulated Microgravity Environment

作　　者：郝彤[1] 王滟濛[1] 李俊杰[1] 杜芝燕[1] 段翠密[1] 王常勇[1] 宋锦苹[2] 王林杰[2] 李莹辉[2] 王妍[1] 

机构地区：[1]军事医学科学院基础医学研究所前沿交叉学科研究室,北京100850 [2]中国航天员科研训练中心,航天医学基础与应用研究国家重点实验室,北京100094

出　　处：《中国实验血液学杂志》2012年第1期182-186,共5页Journal of Experimental Hematology

基　　金：载人航天领域第一批预先研究项目资助,编号010201

摘　　要：本研究旨在探索香菇多糖对抗旋转式细胞培养系统(rotary cell culture system,RCCS)模拟微重力环境下淋巴细胞免疫功能降低的作用。体外分离培养小鼠脾淋巴细胞,在正常重力环境和RCCS模拟微重力环境下分别添加10、20和40μg/ml的香菇多糖溶液,培养24、48和72 h取样,用MTT法、ELISA和流式细胞术分别检测香菇多糖对细胞增殖度、细胞因子分泌量和表面标志表达的影响。结果表明,10、20和40μg/ml香菇多糖在处理时间内对小鼠脾淋巴细胞增殖无明显促进作用;不同浓度的香菇多糖均可提高小鼠脾淋巴细胞在模拟微重力环境下IL-2和IFN-γ的分泌量和促进小鼠脾淋巴细胞表面CD4和CD8分子的表达。结论:香菇多糖具有对抗模拟微重力环境造成的淋巴细胞免疫功能降低的作用。This study was aimed to evaluate the effect of lentinan on the immune function of splenic lymphocytes in rotary cell culture system（RCCS） microgravity enviroment. The splenic lymphocytes from mice were separed and cul- tured in the normal gravity and the microgravity environments. The cells were treated with lentinan solution（0, 10, 20 and 40 p,g/ml）. After incubated with lentinan for indicated times（24, 48 and 72 h）, the cell proliferation, secretion of cytokine and the expression of cell surface markers were detected by MTT method, ELISA and flow cytometry respec- tively. The results indicated that lentinan of above mentioned concentrations did not obviously promote the lymphocyte proliferation, but increased the secretion of IL-2 and IFN-~/ and enhanced the expression of lymphocyte surface markers CD4 and CD8 in microgravity environment. It is concluded that lentinan has the ability to enhance the lymphocyte im- mune function in microgravity environment.

关 键 词：香菇多糖 旋转式细胞培养系统 模拟微重力 淋巴细胞 免疫抑制 

分 类 号：R979.1[医药卫生—药品] R852.22[医药卫生—药学]