CD_(44)基因上皮源性亚型与造血源性亚型mRNA表达之比值与人大肠癌进展转移的关系  

Ratio of CD_(44) Epithelial Variant and CD_(44) Hematopoietic Variant as It Related to Progression and Metastasis of Human Colorectal Cancer

作　　者：高随宽[1] 徐文怀[1] 万远廉[1] 高燕 肖宇 刘占兵 

机构地区：[1]北京医科大学第一医院普外科,北京市100034

出　　处：《中国肿瘤临床》2000年第2期101-103,共3页Chinese Journal of Clinical Oncology

摘　　要：目的 :CD44分子是多种细胞表面的跨膜糖蛋白。有研究表明其变异型如含V6、V8～10 外显子的变异型表达增加与大肠癌进展转移有关。进一步探讨大肠癌组织表达的上皮源性亚型与造血源性亚型之比(E/H)与肿瘤转移等临床资料间的关系。方法 :应用逆转录、多聚酶链反应和Southern杂交技术、检测26例大肠癌患者癌组织和正常大肠粘膜组织CD44 mRNA表达 ,以上皮源性亚型与造血源性亚型之比(E/H)定量。结果 :E/H值癌组织明显高于相应正常粘膜组织 ,但未发现与肿瘤转移、浸润深度等临床资料有关。结论 :CD44上皮源性亚型mRNA在大肠癌组织中表达明显增高 ,E/H值与大肠肿瘤进展转移无明显相关性。上皮源性亚型在肿瘤进展转移中的作用有待深入研究。Objective: CD44 molecule is a transmembrane glycoprotein expressed on various cell surface Recent studies have shown that overexpression of some variant forms such as variants with exon 6 or 8～10 might be involved in tumor progression or metastasis in colorectal cancer In this study, we determined the epithelial variant/hematopoietic variant (E/H-ratio) in human colorectal cancer and investigated its correlation with clinical features such as cancer metastasis Methods: The expression of CD44 mRNA was examined in 52 specimens including cancers and adjacent noncancerous mucosa In 26 patients with colorecxtal cancer, the E/H ratio was calculated to quantitate the level of the epithelial form by reverse transcription-polymerase chain reaction and Southern blot hybridization Results: The E/H ratio in cancerous tissue was significantly higher than that in adjacent noncancerous mucosa;but significant correlations was not observed between the ratio and clinical features Conclusion: Expression of CD44 epithelial form is obviously higher in colorectal cancer tissue than in matched normal mucosa The E/H ratio is not associated with progression and metastasis in colorectal cancer The role of CD44 variant forms in progression and metastasis of colorectal cancer needs to be elucidated

关 键 词：CD44基因 mRNA 大肠肿瘤 E/H值 

分 类 号：R730.3[医药卫生—肿瘤]