多药耐药细胞系HL60/HHT的建立及耐药机理的初步探讨  

ESTABLISHMENT OF THE DRUG RESISTANT CELL LINE HL60/HHT AND RESEARCH ON THE MECHANISM OF ITS MULTIDRUG RESISTANCE

作　　者：薛红漫[1] 李文益[1] 候玲玉 夏焱[1] 郭海霞[1] 

机构地区：[1]广州中山医科大学附属孙逸仙纪念医院儿科

出　　处：《中国小儿血液》2000年第1期13-15,共3页China Child Blood

摘　　要：为探讨HL60/HHT细胞系耐药性的分子机理,采用反复短期暴露法建立HL60/HHT细胞系,并检测其bcl-2和bax的含量。结果表明,建立了多药抗性HL60/HHT细胞,其bcl-2水平为HL60的1.50倍(92.05/61.35),有显著性差异(P<0.01)。其bax蛋白在二种细胞中均低水平表达(HL6015.92,HL/60/HHT17.63),差异无显著意义(P>0.05)。因此。To probe into the molecullar mechanism of drug resistance of HL60/HHT cell lines. the repeat and short term exposing method was used to develop the drug resistant cell lines. Using FITC Conjugated monoclonal mouse antihuman oncoprotein to determine bcl 2 and bax protein in HL60 and HL60/HHT cells. Results show, multidrug resistant HL60/HHT sublines was established. bcl 2 protein level in HL60/HHT was 1 50 times to that in HL60 (92.05/61 35), and there was a significant difference (p<0 01) Bax protein was low level expression in both two cell lines (HL60 15.92, HL60/HHT 17 63). There was no significant difference(p>0 05). Concludes that high level of bcl 2 protein in HL60/HHT cell line is one of the important causes of its nonsensitivity.

关 键 词：儿童 急性 白血病 HL60/HHT 多药耐药性 

分 类 号：R730.2[医药卫生—肿瘤]