MTT法检测大豆异黄酮对癌细胞的生长抑制作用  

The Effect of Soy Isoflavone on Mouse Erythroleukemia Cell Line and Rat Liver Cancer Cell Line By MTT

作　　者：徐春华[1] 

机构地区：[1]大连职业技术学院电气与电子工程技术系,辽宁大连116037

出　　处：《中央民族大学学报（自然科学版）》2012年第1期58-62,共5页Journal of Minzu University of China(Natural Sciences Edition)

基　　金：大连市科技计划项目(No.2009E12SF165)

摘　　要：本文选择小鼠红白血病细胞系CML-K562和大鼠肝癌细胞系CBRH-7919作为大豆异黄酮的作用对象,通过MTT法检测大豆异黄酮对CML-K562和CBRH-7919的抑制作用.结果显示,大豆异黄酮对小鼠红白血病细胞系CML-K562和大鼠肝癌CBRH-7919细胞生长均有抑制作用,其抑制率达到50%时,所需要的用量即IC50分别为15.36 mg/L和16.58 mg/L.Mouse erythroleukemia cell line CML-K562 and Rat liver cancer cell line CBRH-7919 were chosen as the targets of soy isoflavone to study its suppression effects.That detected the efficacity of the soy isoflavone and the inhibition rate cell growth by MTT and cell ectogenesis.It was proved that soy isoflavone could greatly inhibit the growth and proliferation of MEL cell CML-K562（IC50 is 15.36mg/L） and Rat Liver Cancer Cell CBRH-7919（IC50 is 16.58mg/L） by use of MTT.It proved that the soy isoflavone could inhibit the growth of the two cancer cells,and the dose was related with the effectiveness.

关 键 词：大豆异黄酮 生长抑制 MTT法 

分 类 号：R73-36[医药卫生—肿瘤]