马氏钳蝎毒抗癌多肽与5-Fu对小鼠肝癌的联合治疗作用  

Effect of anticancer polypeptide from Buthus martensii venom and its combination with 5-Fu on H_(22)-bearing mice.

作　　者：杨建斌[1] 董伟华[2] 孔天翰 宋红霞[3] 肖鹏[3] 郑智敏 刘桂亭 

机构地区：[1]河南医科大学生物毒素中心 [2]山西省长治医学院病理教研室 [3]山西省长治医学院病理科

出　　处：《中华肝胆外科杂志》2000年第1期17-18,共2页Chinese Journal of Hepatobiliary Surgery

基　　金：河南省计委"九五"重点攻关项目;自然科学基金

摘　　要：目的　探讨马氏钳蝎毒抗癌多肽 (APBMV)单独及其与 5 Fu联合对小鼠肝癌抑瘤作用。方法　采用小鼠腹水型肝癌H2 2 的荷瘤模型观察了APBMV的体内抑瘤作用和对 5 Fu抑瘤作用的影响。结果　APBMV在所用剂量 [(0 .0 3 0 .0 6)mg (kg·d) ,用 10d]范围内对H2 2 荷瘤小鼠无显著的抑瘤作用。 5 Fu 2 0mg (kg·d)用 10d虽有较好的抑瘤效应 ,却有明显毒性反应。将 5-Fu剂量降低 1 2、1 4与APBMV合用则有良好的抑瘤作用 (生命延长率为 10 3 .8%、2 0 .4 % ,P <0 .0 5)且无明显的毒性反应。结论　APBMV可显著增强 5 Fu对肝癌的疗效、降低其毒性。Objective To investigate the antitumor effect of an anticancer polypeptide from Buthus martensii venom (APBMV) and its combination with 5 Fu on H 22 bearing mice.Methods Taking H 22 (ascitic type hepatocellular carcinoma) bearing mice as the animal model, the antitumor activity of APBMV was studied in vivo.Results APBMV administered intraperitoneally at the dosage ranging from 0.03 mg·kg -1 to 0.06 mg·kg -1 once daily for 10 d had no apparent effect on the H 22 bearing mice. 5 Fu had significant efficacy but showed severe toxicity on the mice. However, using APBMV at 0.03 mg·kg -1 combined with 5 Fu at 10 mg·kg -1 , the life prolonging rate was 103.8%(P<0 .001).Conclusions APBMV could enhance the antitumor effect of 5 Fu and antagonize its toxicity markedly.

关 键 词：蝎毒 5-FU 肝肿瘤 APBMV 小鼠 

分 类 号：R73-36[医药卫生—肿瘤]