凋亡基因bcl-2/bax蛋白在胆囊良、恶性病变中表达及其临床意义  

Expression and clinical significance of bcl-2/bax protein in benign and malignant lesions of the gallbladder

作　　者：郝秀原[1] 石景森[2] 周连锁[2] 王作仁[2] 卢云[2] 田和平[2] 韩梅荣 

机构地区：[1]山西省人民医院普外科,太原030012 [2]西安医科大学第一临床医学院肝胆外科研究室,西安710061

出　　处：《西安医科大学学报》2000年第1期31-33,共3页Journal of Xi'an Medical University(Chinese)

摘　　要：选取胆囊炎标本 16例 ,胆囊癌标本 4 0例行免疫组织化学 (S- P法 ) ,对凋亡相关基因 bcl- 2与bax染色。结果表明胆囊炎组、胆囊癌组及癌旁组织 bcl- 2阳性率分别为 4 3.7%、77.5%与 81.8% ,胆囊癌组高于胆囊炎组 (P <0 .0 5) ,与癌旁组织相比无显著性差异 (P >0 .0 5)。bax染色示胆囊炎组、胆囊癌组和癌旁组织阳性率分别为 81.5%、52 .5%和 54.5% ,胆囊癌组与胆囊炎组相比差异显著 (P<0 .0 5)。癌旁组织与胆囊炎组及胆囊癌组相比差异不显著。胆囊癌 bcl- 2阳性率与分化程度有关 ,Bax在胆囊癌不同分化程度的表达率不同 ,bcl- 2与 bax相互表达具有一定联系 (0 .0 5<P <0 .1)。表明凋亡 ,特别是 bcl- 2家族在胆囊癌发病中可能具有作用。To explore the functioning of bcl 2 gene family in biological behavior in benign and malignant lesions of the gallbladder,bcl 2 and Bax protein levels were assessed by immunohistochemical methods(S P) in 16 specimens of cholecystitis and 40 of PCG.Statistical comparisons of immunostaining results with clinical and pathological features were performed.The results showed that the percentages of detectable bcl 2 expression shown in PCG(77.5%) and adjacent dysplastic lesions were significantly higher than that in cholecystitis (P<0.05).Enhanced bcl 2 expression in PCG wasnt correlated with histological subtypes, sex ,age, complicated gallstones and stage, but with grading.The percentage of detectable bax expression shown in cholecystitis was significantly higher than that in PCG(0.025<P<0.05).The positive rate of bax in adjacent dysplastic lesions was not significantly different from PCG and cholecystitis.The expression of bax in PCG correlate with grade, but not with other clinical and pathological features, bcl 2 is negatively correlated with expression of bax.Apoptosis, especially the bcl 2 gene family ,proves likely to be a tumorigenesis factor for PCG.

关 键 词：胆囊肿瘤 免疫组织化学 BCL-2/BAX蛋白 

分 类 号：R735.802[医药卫生—肿瘤]