纳米Fe_3O_4对小鼠肺细胞的氧化损伤  

Oxidative Damage of Mouse Lung Cells Induced by Fe_3O_4 Nanoparticles

作　　者：马萍[1,2] 杜娟[2] 罗清[2] 赵静云[2] 尤会会[2] 杨旭[2] 

机构地区：[1]咸宁学院基础医学院细胞生物学与遗传学教研室,咸宁437100 [2]华中师范大学生命科学学院环境科学实验室,武汉430079

出　　处：《生态毒理学报》2012年第1期44-48,共5页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金面上项目(No.50802032)

摘　　要：纳米Fe3O4作为一种功能材料,在生物医药、生物靶向材料、微波吸收材料和高梯度磁分离器等方面应用前景广阔,其潜在的生物毒性也备受关注。为研究纳米Fe3O4对生物体可能造成的氧化损伤,以昆明小鼠为受试体,设置5、10、20和40mg·kg-14个染毒组,腹腔注射染毒7d后,测定小鼠肺组织中活性氧(reactive oxygen species,ROS)、还原型谷胱甘肽(glutathione,GSH)和丙二醛(malondialdehyde,MDA)的含量。结果显示,随着纳米Fe3O4染毒剂量的升高,肺组织ROS和MDA含量逐渐上升,GSH含量逐渐降低,各指标均呈一定的剂量-效应关系。剂量≥10mg·kg-1,肺组织ROS含量与对照组相比有显著差异(p<0.05);剂量≥20mg·kg-1,肺组织MDA含量与对照组相比有显著差异(p<0.05);剂量≥40mg·kg-1,肺组织GSH含量与对照组相比有显著差异(p<0.05)。研究表明,较高剂量(≥20mg·kg-1)的纳米Fe3O4颗粒材料会引起小鼠肺细胞的氧化损伤。As an important plasticizer for polyvinyl chloride plastics,DEHP was widely used in industry,which was currently detected in the ocean,air,drinking water,animals and plants to different extent. DEHP has caused ever-increasing ecological environment pollution. In this paper,the domestic and foreign toxicological researches were reviewed,which were related to the human exposure and metabolic pathways of DEHP. The toxicity and potential danger of DEHP to animals and human health were discussed in detail from the aspect of liver,heart,reproductive/developmental system and respiratory system. On these bases,the existing problems and further research directions were pointed out.

关 键 词：纳米FE3O4 小鼠 肺 氧化损伤 

分 类 号：X171.5[环境科学与工程—环境科学] R12[医药卫生—环境卫生学]