茶多酚对大鼠全脑缺血／再灌注损伤后神经元凋亡和神经行为学的影响  

The impact of polyphenols on the hippocampal neuronal apoptosis and ethology of rats after global cerebral ischemiareperfusion injury

作　　者：赵静[1] 薛荣亮[1] 魏欣[1] 付荣国[1] 吕建瑞[1] 田俊斌[1] 吴刚[1] 李伟[1] 雷小明[1] 

机构地区：[1]西安交通大学第二附属医院麻醉科,710004

出　　处：《国际麻醉学与复苏杂志》2012年第3期146-150,共5页International Journal of Anesthesiology and Resuscitation

基　　金：国家自然科学基金（81071070）

摘　　要：目的观察茶多酚对大鼠全脑缺血，再灌注（ischemia／reperfusion，I／R）损伤后海马CA1区神经元凋亡和神经行为学改变的影响。方法健康清沽雄性SD大鼠216只，按随机数字表法分为3组（每组72只），分别为假手术组（sham operation，SO组），缺血，再灌注模型组（I／R组）和茶多酚组（teapolyphenols，TP组，TP200mg／kg再灌注即刻腹腔注射），采用DNA断裂的原位末端标记法（UNEL法）以及流式细胞仪检测细胞凋亡，利用旷场实验和高架十字迷宫实验评价神经行为学变化。结果大鼠全脑I／R后，TUNEL法结果光镜下观察显示：I／R组海马CA1区可见较多凋亡细胞，TP组凋亡细胞数较I／R组明显减少，流式细胞仪检测结果显示：全脑I／R后48h，TP组细胞凋亡率18．77％，I／R组细胞凋亡率达26．47％，TP组与I／R组相比明显降低，两组间差异有统计学意义（P〈0．05）。旷场试验和高架十字迷宫实验结果显示：与I／R组比较，TP组大鼠探索能力增强，焦虑情绪减轻，差异有统计学意义（P〈O．05）。结论大鼠全脑I／R后，茶多酚能够发挥抗凋亡作用，降低海马CA1区神经元的凋亡率，并在抑制神经元凋亡基础上改善大鼠全脑I／R后的神经行为学功能。Objective Observe the influence of tea polypheno]s on hippoeampal neuronal apoptosis in CA1 region of rats after global cerebral isehemia/reperfusion （I/R） injury, and discuss the relationship between rat＇s neuronal behavior and hippocampal neuronal apoptosis in addition. Methods 216 healthy male SD rats were randomly divided into 3 groups： sham operation group （SO group, n=72）, ischemia/reperfusion group （I/R group, n=72） and tea polyphenols group （TP group, n=72, TP 200 mg/kg, TP were intraperitoneally injected at the time of reperfusion）, TUNEL was used to observe, qualitative and locate apoptotic neurons, flow cytometry was used to detected apoptotic index. The open field test and the high cross maze were used to test the rats~ ueuroethology. Results After cerebral ischemia/reperfusion injury, TUNEL assay showed that： compared with TP group, hippocampal neurons in CA1 region of I/R group can be seen more apoptotie cells. Apoptotie cells tested by flow cytometry showed： the apoptosis of TP group reached 26%, compared with the I/R group, the rate of apopotosis was significantly reduced （P〈0.05）. Open-field test and elevated plus maze experiments showed that： compared with I/R group, TP group showed some activity and the ability to explore and their emotions are more stable （P〈0.05）. Conclusions After cerebra] isehemia/reperfusion, tea polyphenols can play the role of anti-apoptnsis, reducing the rate of cellular apoptosis in hippocampal CA1 region and it was. able to improve rats＂ neurological ethology based on this function.

关 键 词：脑 缺血/再灌注损伤 行为学 凋亡 茶多酚 

分 类 号：R614[医药卫生—麻醉学]