原发性肝癌患者血清AFP及外周血AFP mRNA结果分析  

Analysis the Results on Detection of Serum AFP and AFP mRNA in the Peripheral Blood of Patients with Primary Liver Cancer

作　　者：骆耐香[1] 彭德珍[2] 刘菁[1] 陈森洲[1] 袁桂峰[1] 徐雅娟[1] 

机构地区：[1]桂林医学院临床微生物学与免疫学教研室,541004 [2]桂林医学院附院检验科,541001

出　　处：《放射免疫学杂志》2012年第1期73-75,共3页Journal of Radioimmanology

基　　金：广西教育厅科研项目(项目编号200911LX296)

摘　　要：目的:探讨血清AFP及外周血AFP mRNA联检对原发性肝癌(PLC)患者的早期诊断和微小转移的临床意义。方法:采用电化学发光法和实时荧光定量PCR分别检测40例PLC患者血清AFP和外周血AFPmRNA。结果:PLC患者血清AFP和外周血AFP mRNA阳性率分别为72.50%(29/40)和57.50%(23/40),显著高于肝硬化患者(8.33%和4.17%,P<0.05)。其中血清AFP阳性和阴性的PLC患者分别有21例(72.41%)和2例(18.18%)检出AFP mRNA。结论:外周血AFP mRNA可作为血循环中存在肝癌细胞的一个早期标志物,与血清AFP联检有助于提高PLC的确诊率,更好地预测肿瘤转移和复发。同时为开展PLC靶向治疗提供依据。Objective To explore the clinical significance of early diagnosis and tiny metastasizing on patients with primary liver cancer （PLC） by combined detection of serum AFP （alpha fetal protein） and AFP mRNA in the peripheral blood. Methods The serum AFP and expression of AFP mRNA in the peripheral blood of 40 patients with PLC were tested by eleetroehemilumineseence assay and real-time fluorescent quantitative RT-PCR, respectively. Results The positive rates of serum AFP and AFP mRNA in the peripheral blood of PLC patients were 72.50% （29/40） and 57.50% （23/40）, separately. It was significantly higher than that of patients with liver cirrhosis （ 8.33 % and 4.17 %, P 〈 0.05 ）. There were 21 eases （72.41% ） and 2 eases （ 18.18 % ） tested the expression of AFP mRNA among serum AFP positive and negative, individually. Conclusion AFP mRNA in the peripheral blood can be used as an early marker of liver cancer cells in blood circulation. It is helpful to improve definite rate of PLC, and preferably predict the risk of tumor metastasizing and recurrence. It can also provide the reliable evidence for PLC targeted therapy.

关 键 词：原发性肝癌 甲胎蛋白 实时荧光定量PCR 电化学发光法 

分 类 号：R735.7[医药卫生—肿瘤]