SD大鼠胆汁淤积性肝纤维化动物模型的建立与评价  

Establishment and evaluation of cholestatic liver fibrosis model induced by bile duct ligation in SD rat

作　　者：刘迎春[1] 顾小红[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所高干科,重庆400042

出　　处：《重庆医学》2012年第5期458-460,F0003,共4页Chongqing medicine

基　　金：重庆市自然科学基金资助项目(CSTC2010BB5165);中国人民解放军军第三军医大学中青年科学基金资助项目(XG200543)

摘　　要：目的通过胆管结扎法建立胆汁淤积性肝纤维化动物模型,并对模型进行评价与探讨。方法选用禁食12h、能自由饮水的SD雄性大鼠,腹腔麻醉后,分离胆管,以丝线两处剪断1cm结扎胆管,分别于0、2、4、6、8周测定大鼠体质量、肝脏质量、肝功能、肝纤维化酶谱,镜下观察肝脏组织学及超微结构的改变,并设立假手术组和对照组。结果 4～6周后,模型组大鼠体质量及肝脏质量较假手术组和对照组明显下降(P<0.05),肝脏湿质量明显下降(P<0.05),造模第2周即可见肝功能及肝纤维化酶谱较0周时明显升高(P<0.05),病理切片可见肝组织结构明显破坏,大量炎细胞浸润、胶原纤维增生及假小叶形成。结论胆汁淤积性肝纤维化模型造模方法简单,形成周期短,是一种稳定且可靠的肝纤维化研究模型。Objective To establish and evaluate the cholestatic liver fibrosis model induced by bile duct ligation in SD rat.Methods The bile ducts of the rats were occluded by ligation.Serum AST,ALT,TB,HA and LN were determined in 0,2,4,6,8 weeks separately,as well as body weight,liver weight and the changes of the liver histology and ultrastructure.A sham operation and normal group were used as control.Results After 2 weeks,serum AST,ALT,TB,HA and LN levels of the model group were much higher than those of the control group.After 4-6 weeks,body weight and liver weight were decreased significantly,amount of inflammatory cells infiltration in damage liver structure,collagen fiber hyperplasia and false flocculus were formed.Conclusion The model of liver cholestatic fibrosis induced by bile duct ligation is an ideal study model of liver fibrosis,because it is model-constructing method is simple,short for processing cycle,stable and harmless to human and enviroment.

关 键 词：大鼠 肝硬化 动物模型 结扎胆管 

分 类 号：R575.2[医药卫生—消化系统]