一株高产低温纤维素酶南极细菌的筛选、鉴定及酶学性质初步研究  

Screening,Identification and Characterization of A High-Producing Cold-Adapted Cellulase Antarctic Bacterium

作　　者：高丛[1] 缪锦来[2] 郑洲[2] 金青[1] 

机构地区：[1]青岛科技大学化工学院,山东青岛266042 [2]国家海洋局第一海洋研究所,山东青岛266061

出　　处：《化学与生物工程》2012年第2期37-39,46,共4页Chemistry & Bioengineering

基　　金：国家自然科学基金资助项目(40876107)

摘　　要：从82株南极细菌中筛选出1株高产低温纤维素酶菌株NJ64,通过16S rDNA序列分析对其进行鉴定,并对其产酶条件及酶学性质进行了初步研究。结果表明,NJ64菌株为假交替单胞菌属(Pseudoalteromonas sp.);最适产酶条件为:培养时间72 h、初始pH值7.5、培养温度10℃;所产纤维素酶在pH值9.0左右、40℃条件下的酶活性最高,属于低温酶。A high-producing cold-adapted cellulase antarctic bacterium named NJ64 was isolated from 82 antractic bacteria and identified by 16S rDNA sequence analysis. Its cellulase-producing conditions and enzymatic properties were studied primarily. The strain NJ64 was determined as genus Pseudoalteromonas sp.. The optimum cellulase-producing conditions were obtained as follows：cultural time was 72 h,initial pH value was 7.5, cultural temperature was 10 ℃. The enzymatic activity of cold-adapted cellulase was the highest with pH value of about 9. O,temperature of 40 ℃.

关 键 词：南极 细菌 低温纤维素酶 

分 类 号：TQ920[轻工技术与工程—发酵工程] Q936[生物学—微生物学]