平榛冷适应相关基因CBF的克隆及时空表达特性分析  

Cloning and Temporal-Spatial Expression of a CBF Homolog Associated with Cold Acclimation from Corylus heterophylla

作　　者：陈新[1] 王贵禧[1] 梁丽松[1] 马庆华[1] 

机构地区：[1]中国林业科学研究院林业研究所林木遗传育种国家重点实验室,北京100091

出　　处：《林业科学》2012年第1期167-172,共6页Scientia Silvae Sinicae

基　　金：国家"十一五"科技支撑计划"高产优质大枣;榛子新品种选育"(2006BAD01A1701);国家林业局"948"项目"榛子特异性状育种材料及微繁技术引进"(2008204208)

摘　　要：低温是影响植物分布、进而影响生长和产量的关键因素之一。Weiser（1970）指出低温导致植物的基因表达发生变化;早在20世纪90年代研究者就发现了植物的冷适应现象（Guyetal.,1985）。虽然迄今为止植物抗寒的分子机制还没完全清楚。CBF transcription factors play a central role in controlling freezing tolerance in plants. A novel CBF/DREB1 gene was isolated, from Corylus heterophylla, one of the cold-hardiest Corylus species, named as ChCBF. Sequence analysis revealed that ChCBF had 1 053 bp in full length and contained an open reading frame （ORF） of 684 bp encoding a 227 predicted amino acids residues which possessed conserved AP2/EREBP domain for the CBF function. Sequence alignment and phylogenetic analysis revealed that conserved domain of ChCBF shared high identity with CBF homologues from other plant species in all conserved regions. Expression profiling of ChCBF was performed by qRT-PCR method, ChCBF transcripts were highest at the stage of cold acclimation （CA）, while the level of ChCBF was down-regulated during the coldest mid-winter （MW）, and increased again as buds deacclimated （DA）. ChCBF transcripts were rapidly and highly induced when plants were exposed to low temperatures of 4 ℃, and ChCBF was activated in 4 h reaching a peak, suggesting that the critical transcriptional changes of ChCBF occurred majorly during the initial 4 hours and it was possibly involved in early-stage responses to low temperature. Spatial expression analyses revealed that the transcription level of ChCBF was relatively higher in bud than that in male inflorescence and bark tissues. These results suggest that ChCBF may participate in the cold acclimation process and show temporal-spatial specific expression characteristics in hazelnut.

关 键 词：平榛 CBF 荧光定量PCR 时空表达特性 

分 类 号：S718.46[农业科学—林学]