固相萃取-高效液相色谱法同时测定食品塑料包装材料中9种光稳定剂  

Simultaneous determination of 9 ultraviolet stabilizers in food plastic packaging materials by solid phase extraction-high performance liquid chromatography

作　　者：张居舟[1] 李静[1] 邵栋梁[1] 姚帮本[1] 蒋俊树[1] 

机构地区：[1]安徽国家农业标准化与监测中心国家农副加工食品质量监督检验中心,安徽合肥230051

出　　处：《色谱》2012年第2期190-195,共6页Chinese Journal of Chromatography

基　　金：国家质量技术监督检验检疫总局科技计划项目(Nos.2009QK190;2009QK191);安徽省质量技术监督局科研计划项目(No.ZJY-013)

摘　　要：建立了同时测定食品塑料包装材料中9种紫外光稳定剂含量的高效液相色谱方法。样品用甲醇-乙酸乙酯混合溶剂超声提取,经固相萃取小柱净化后,以ZORBAX SB-C18柱(250 mm×4.6 mm,5μm)为分离色谱柱,甲醇和水为流动相,梯度洗脱,以310 nm为检测波长进行定性、定量分析。该方法前处理简单、易操作,9种紫外光稳定剂分离效果良好。9种紫外光稳定剂在0.2～10 mg/L范围内呈良好的线性关系,线性相关系数大于0.999;方法检出限为0.05～0.1 mg/L;实际样品中的加标回收率为70.2%～89.0%,相对标准偏差为0.4%～4.5%。该方法简单、准确,能够满足食品塑料包装材料中紫外光稳定剂的检测要求。An effective high performance liquid chromatographic （HPLC） method has been developed for the simultaneous determination of 9 ultraviolet stabilizers in food plastic packa- ging materials. The food packaging samples were firstly extracted by methanol-ethyl acetate, and then purified by a Cl8 solid-phase extraction （SPE） colunm. The target compounds were separated on a ZORBAX SB-C18 column （250 mm x4.6 ram, 5 ~m） in gradient elution mode using methanol and water as mobile phases. The detection wavelength was at 310 nm. The line- ar plots of the nine ultraviolet stabilizers were obtained between 0. 2 and 10 rag/L, with the cor- relation coefficients of above 0. 999 for the nine ultraviolet stabilizers. The limits of detection for this method were in the range from 0.05 to 0. l mg/L. The recoveries spiked in commercial food plastic packaging materials were in the range of 70.2% - 89.0% with the relative standard deviations of 0.4% - 4.5%. The results indicated that the method is simple, accurate, and suitable for the simultaneous determination of the nine ultraviolet stabilizers in food plastic packaging materials.

关 键 词：高效液相色谱 紫外光稳定剂 塑料包装材料 食品 

分 类 号：O658[理学—分析化学]