氧增强放疗对大鼠胶质瘤细胞凋亡相关基因Bcl-2和Bax影响  

Impact of radio-therapy after hyperbaric oxygenation on apoptosis-related gene Bcl-2 and Bax expressions of glioma in the rat

作　　者：胡殿雷[1] 李伯安[1] 王余军[1] 戴如飞[2] 阎超[2] 

机构地区：[1]徐州医学院附属第三医院神经外科,221003 [2]徐州医学院附属第二医院神经外科

出　　处：《临床神经外科杂志》2011年第6期296-297,共2页Journal of Clinical Neurosurgery

摘　　要：目的探讨氧增强放疗对胶质瘤凋亡相关基因Bcl-2、Bax影响的差异。方法建立大鼠胶质瘤动物模型,治疗组10只,高压氧处理后放疗;对照组10只,常规放疗。免疫组化检测凋亡相关基因Bcl-2、Bax的表达。结果治疗组Bcl-2表达明显低于对照组,组问差异有显著性(P<0.05);治疗组Bax的表达明显高于对照组,组间差异有显著性(P<0.05)。结论氧增强放疗可以更有效地抑制Bcl-2、增加Bax的表达。Objective To investigate the impact of radio-therapy after hyperbaric oxygenation on apoptosis-related gene of Bcl-2, Bax expressions in glioma. Motheds The glioma models were established in 20 SD rats, and were divided into two groups ： treatment group （ 10 rats, treated with radio-therapy combided with hyperbaric oxygenation） , control group （10 rats, treated by common radio-therapy） o When treatment was finished, the expressions of Bcl-2, Bax were detected by immunostaining assay using Streptavidin-Peroxidase （SP） mothed. Results The expression of Bel-2 protein in treatment group significantly lower than the control group. There were significantly differences between the two groups （ P 〈 0.05 ） ; The expression of Bax protein in treatment group significantly higher than the control group. There were significantly differences between the two groups（P 〈 0.05 ）. Conclusions Rradio-therapy after hyperbaric oxygenation can more effectly inhibit the expression of the Bcl-2 and increase the expression of the Bax.

关 键 词：高压氧 放疗 胶质瘤 细胞凋亡 癌基因蛋白 

分 类 号：R739.41[医药卫生—肿瘤]