唑来膦酸局部应用对大鼠拔牙创软硬组织的影响  

Study of application of zoledronic acid locally affects hard and soft tissue of the extraction socket in rats

作　　者：崔彩雯[1] 张健[1] 马婷[1] 汪铭[2] 

机构地区：[1]天津医科大学口腔医院颌面外科,硕士生天津300070 [2]南京市中西医结合医院,医师江苏210000

出　　处：《口腔颌面修复学杂志》2011年第5期261-264,共4页Chinese Journal of Prosthodontics

摘　　要：目的:观察唑来膦酸(zoledronic acid,ZA)局部应用对大鼠下颌磨牙拔除后剩余软硬组织的影响。方法:将54只雄性Wistar大鼠随机分为三组,即空白对照组、明胶海绵组和唑来膦酸治疗组,麻醉下完整拔除左侧下颌第一磨牙并做相应处理,术后2周同期处死所有大鼠。用X线片测量大鼠拔牙窝剩余牙槽嵴的高度,取拔牙窝周围牙龈组织制作石蜡切片行HE染色和Masson染色,观察大鼠下颌磨牙拔牙窝的愈合情况。结果:唑来膦酸治疗组牙槽嵴高度显著高于空白对照组和明胶海绵组(2.60±0.10mmVS1.99±0.22mm,P<0.001;2.60±0.10mm VS 2.12±0.24mm,P<0.001)。组织学观察拔牙创周围黏膜层上皮变薄,胶原较为纤细且排列疏松,血管变化不明显。结论:唑来膦酸局部应用抑制牙槽嵴骨吸收,促进拔牙窝新骨形成,对软组织作用不显著,可能抑制牙龈黏膜中胶原纤维的合成。Objective： To investigate the effects of locally application of zoledronic acid（ZA） on hard and soft tissue in rats that were pretreated with ZA during tooth extraction.Methods： Fifty-four 7-week old male Wistar rats were divided into ZA group,gelatin sponge group,and blank control group.In each group,left mandibular first molars were extracted under general anesthesia.Rats were euthanized two weeks after tooth extraction,and mandibular bones were collected.Bone morphometric analysis using X-ray images was performed and the gingival around the extraction socket was harvested for HE staining and Masson staining to observe the histopathology effect.Results： New bone formation in the extraction socket was promoted and alveolar ridge absorption was delayed in the ZA group compared to the control group.The alveolar ridges of the ZA group were higher than those of the control group（2.60±0.10mmVS1.99±0.22mm,P 0.001;2.60±0.10mm VS 2.12±0.24mm,P 0.001）.Histological images in ZA group showed that the mucous about extraction socket was getting thinner,the fiber was slender and disordered,but no differences of blood ressels were observed.Conclusions： Local application of zoledronic acid could inhibit bone resorption and promote new bone formation of the tooth extraction socket,also it might inhibit the formation of collagen fibers.These actions of ZA may be relevant to the pathogenesis of BRONJ.

关 键 词：唑来膦酸 局部用药 牙槽骨 软组织 

分 类 号：R783[医药卫生—口腔医学]