基于PCR标记的陕西省部分地方小麦品种(系)糯蛋白基因的多样性分析  

A PCR-Based Diversity Analysis of Waxy Genes in Shaanxi Wheat Cultivars and Lines

作　　者：杨松杰[1,2] 张晶[3] 张晓科[3] 

机构地区：[1]安康学院农学与生命科学院,陕西安康725000 [2]陕西省富硒食品工程实验室,陕西安康725000 [3]西北农林科技大学农学院,陕西杨凌712100

出　　处：《华北农学报》2011年第6期67-72,共6页Acta Agriculturae Boreali-Sinica

基　　金：陕西省教育厅项目(2008JK212);安康学院科技创新团队项目(2008AKXY006)

摘　　要：利用3个优化的STS标记对陕西省1960年以来引进和自育的99份小麦品种(系)的Waxy蛋白基因的变异情况进行了鉴定。结果表明,Wx-A1位点的标记在Wx-A1a和Wx-A1b基因型内分别扩增出336 bp和317 bp特异片段;Wx-B1a基因型内扩增出425 bp特异产物,Wx-B1b基因型内不扩增出此产物;Wx-D1a和Wx-D1b基因型内分别扩增出867 bp和279 bp特异产物。检测出除陕糯1号基因型为Wx-A1b+Wx-B1b+Wx-D1b外,Wx-B1b基因缺失突变材料12份,无Wx-A1b基因和Wx-D1b基因缺失突变材料。这3个标记可以用于分子标记辅助育种,并提供了部分品种的缺失类型。Three optimized STS markers were used to analyze 99 wheat cultivars in Shaanxi from 1960 with different types of Waxy proteins.The results indicated that the marker on Wx-A1 locus amplified 336 bp and 317 bp special fragments associated to Wx-A1a and Wx-A1b genotypes;the marker on Wx-B1 amplified 425 bp special fragment associated to Wx-B1a and null special fragment associated to Wx-A1b genotype and the marker on Wx-D1 amplified 867 bp and 279 bp special fragments associated to Wx-D1a and Wx-D1b genotypes,respectively.Except for the cultivar Shaannuo 1 with the genotype of Wx-A1b ＋ Wx-B1b ＋ Wx-D1b,12 cultivars were identified to null Wx-B1 mutants and no cultivars were detected for null Wx-A1 mutants and null Wx-D1 mutants.The three optimized STS markers were demonstrated to be useful tools to identify wheat cultivars with mutant and normal alleles of the Waxy genes in marker-assisted selection of wheat breeding programs and the genotypes of Waxy genes of some materials were released in this paper.

关 键 词：普通小麦 地方品种 Waxy蛋白基因 PCR标记 多样性 

分 类 号：Q78[生物学—分子生物学]