双向电泳检测奶牛乳腺上皮细胞核磷酸化蛋白质方法的建立  

Establishment of a Two-Dimensional Gel Electrophoresis Method to Detect Nuclear Phosphorylated Proteins in Dairy Cow Mammary Gland Epithelial Cells

作　　者：黄建国[1] 高学军[1] 李庆章[1] 陆黎敏[1] 骆超超[1] 王佳丽[1] 刘荣[1] 乔彬[1] 金鑫[1] 

机构地区：[1]东北农业大学乳品科学教育部重点实验室,黑龙江哈尔滨150030

出　　处：《乳业科学与技术》2011年第6期250-253,共4页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

基　　金：国家"973"计划项目(2011CB100804)

摘　　要：为研究不同激素或营养素对乳腺上皮细胞核磷酸化蛋白质的影响,本实验建立细胞核磷酸化蛋白质提取方法及双向电泳实验技术。结果表明,细胞核磷酸化蛋白纯化结合双向电泳技术是乳腺上皮细胞核磷酸化蛋白质组学研究的有效方法,可为进一步研究乳腺上皮细胞机理提供技术基础。This paper describes procedures for the extraction,purification and 2-DE gel electrophoresis analysis of nuclear phosphorylated protein,with the aim of providing a basis for studying the influences of hormones and nutrients on nuclear phosphorylated proteins in dairy cow mammary gland epithelial cells（DCMGECs）.The established procedures proved effective approaches to proteomic analysis of nuclear phosphorylated proteins in DCMGECs and could provide a technical basis for further studies on the lactation mechanism of DCMGECs.

关 键 词：细胞核 磷酸化蛋白质 双向电泳 奶牛 乳腺上皮细胞 

分 类 号：S823.91[农业科学—畜牧学]