微波辅助水解比色法快速检测羊奶粉中水解动物蛋白  

Rapid Determination of Adulteration of Hydrolyzed Animal Protein in Goat Milk Powder by Microwave-Assisted Hydrolysis and Colorimetric Analysis

作　　者：葛娜 葛武鹏[1] 张静[1] 秦立虎 刘志宏[3] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]西安奶业科学研究所,陕西西安710065 [3]新疆农业职业技术学院,新疆昌吉831100

出　　处：《乳业科学与技术》2011年第6期264-267,共4页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

基　　金：西北农林科技大学人才基金资助项目(Z111020923)

摘　　要：目的:寻求一种快速、高效测定羊奶粉中是否添加水解动物蛋白(HAP)的方法。方法:以L-羟脯氨酸为水解动物蛋白特征物,样品通过微波辅助水解处理,测定样品羟脯氨酸的含量变化,并与传统湿法水解对比。结果:微波辅助水解条件:硫酸浓度4mol/L,功率720W,时间45min时,HAP及掺假合成品水解后测得的羟脯氨酸含量与传统湿法水解所测得的羟脯氨酸含量基本相同,RSD为-4.5%～1.6%。本方法可以快速判定羊奶粉中是否添加水解动物蛋白,具有较高准确度和重现性,羟脯氨酸含量与吸光度具有良好的线性关系,决定系数R2=0.9968,加标回收率86.24%～91.30%,可以满足比色法测定结果要求。结论:微波辅助水解可以将样品前处理时间从16h缩短到45min,此方法快速简便,重复性好,回收率高。Objective： To develop a fast and effective method for determining whether goat milk powder is adulterated with hydrolyzed animal protein（HAP）.Methods： L-Hydroxyproline was used as an indicator of the existence of HAP.Samples were hydrolyzed under the assistance of microwave before colorimetric determination of L-hydroxyproline.The microwave-assisted hydrolysis process was compared with the conventional wet hydrolysis process.Results： The optimal microwave-assisted hydrolysis conditions of sulphuric acid concentration microwave power and hydrolysis time were 4 mol/L,720 W,and 45 min,respectively.Substantially identical results for the determination of L-hydroxyproline content in HAP and synthetic products adulterated with L-hydroxyproline were obtained from both hydrolysis processes with a RSD between-4.5% and 1.6%.The method allowed rapid determination of whether goat milk powder is adulterated with HAP,with high accuracy and reproducibility.Moreover,an excellent linear relationship was found between L-hydroxyproline concentration and absorbance with a determination coefficient of 0.9968.Spike recoveries between 86.24% and 91.30% were obtained.Thus,the requirements for the colorimetric determination of L-hydroxyproline were met.Conclusions： Microwave-assisted hydrolysis can shorten the sample pretreatment time from 16 h to 45 min.The method offers the advantages of rapidity,simplicity,good reproducibility and high recovery.

关 键 词：水解动物蛋白 微波水解 L-羟脯氨酸 羊奶粉 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]